新型固相微萃取膜的制备及其在生物检材中毒物提取的应用  

Preparation of a Novel Solid Phase Micro-extraction Membrane and Its Application in Extracting Toxicant from Biological Materials

作　　者：杨瑞琴[1] 谢文林[2] 

机构地区：[1]中国人民公安大学刑事科学技术系,北京100038 [2]吉林省公安厅公安科学技术研究所,吉林长春130051

出　　处：《分析测试学报》2004年第2期66-68,共3页Journal of Instrumental Analysis

基　　金：公安部资助项目 (20002224201)

摘　　要：用酰胺类化合物和气相色谱固定液制备了一种新型固相微萃取膜 ,对膜的性质、吸附解吸条件进行了研究 ;应用该类固相微萃取膜成功地分离了尿液中的吗啡、苯巴比妥以及血液中的敌敌畏 ,并利用气相色谱 -质谱联用技术对分离后的样品进行了分析。Solid phase micro-extraction membrane(SPMEM) is a new, simple and solventless sample preparation technique which intergrates sampling, extraction and concentration into a single step. It has the advantages of both the solid phase micro-extraction(SPME) and membrane separation. In this paper, a new kind of membrane used for SPME was prepared with amide compounds. The extraction conditions such as adsorption time, desorption solvents, desorption methods and time were studied and optimized. The morphine and phenobarbital in the urine, DDVP in the blood were well separated with this SPME membrane, and were examined by GC-MS. All the data were acquired in scan mode except that of morphine which was obtained in a selected ion monitoring(SIM) mode. Ions used for identification were those with m/z 57, 115, 162, 215, 285.

关 键 词：固相微萃取膜 制备 生物检材 毒物 提取 刑事技术 尿液 吗啡 苯巴比妥 血液 敌敌畏 气相色谱-质谱联用技术 

分 类 号：D919.2[医药卫生—法医学]