Effect of staurosporine on cycle of human gastric cancer cells  

作　　者：Min-WenHa Ke-ZuoHou Yun-PengLiu YuanYuan 

机构地区：[1]CancerInstituteoftheFirstAffiliatedHospitalofChinaMedicalUniversity,Shenyang110001,LiaoningProvince,China [2]DepartmentofOncologyoftheFirstAffiliatedHospitalofChinaMedicalUniversity,Shenyang110001,LiaoningProvince,China

出　　处：《World Journal of Gastroenterology》2004年第2期161-166,共6页世界胃肠病学杂志（英文版）

基　　金：Supported bYThe China State Key Basic Research Program,No.G1998051203

摘　　要：AIM:To study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901.METHODS:Cell proliferation was evaluated by trypan blue dye exclusion method.Apoptotic morphology was observed under a transmission electron microscope.Changes of cell cycle and apoptotic peaks of cells were determined by flow cytometry. Expression of p21^WAF1 gene was examined using immunohistochemistry and RT-PCR.RESULTS:The growth of MGC803 and SGC7901 cells was inhibited by ST.The inhibitory concentrations against 50% cells (IC50) at 24 h and 48 h were 54ng/ml and 23ng/ml for MGC803, and 61ng/ml and 37ng/ml for SGC7901.Typical apoptotic bodies and apoptotic peaks were observed 24h after cells were treated wth ST at a concentration of 200ng/ml.The percentage of cells at G0/G1 phase was decreased and that of cells at G2/M was increased significantly in the group treated wth ST at the concentrations of 40ng/ml,60ng/ml,100ng/ml for 24h,compared with the control group (P<0.01).The expression levels of p21^WAF1 gene in both MGC803 and SGC7901 cells were markedly up-regulated after treatment with ST.CONCLUSION:ST can cause arrest of gastric cancer cells at G2/M phase,which may be one of the mechanisms that inhibit cell proliferation and cause apoptosis in these cells.Effect of ST on cells at G2/M phase may be attributed to the up-regulattion of p21^WAF1 gene.AIM:TO study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901. METHODS:Cell proliferation was evaluated by trypan blue dye exclusion method.Apoptotic morphology was observed under a transmission electron microscope.Changes of cell cycle and apoptotic peaks of cells were determined by flow cytometry.Expression of p21^(WAFl)gene was examined using immunohistochemistry and RT-PCR. RESULTS:The growth of MGC803 and SGC7901 cells was inhibited by ST.The inhibitory concentrations against 50% cells (IC_(50)) at 24 h and 48 h were 54 ng/ml and 23 ng/ml for MGC803,and 61 ng/ml and 37 ng/ml for SGC7901.Typical apoptotic bodies and apoptotic peaks were observed 24 h after cells were treated wth ST at a concentration of 200 ng/ml.The percentage of cells at G_0/G_1 phase was decreased and that of cells at G_2/M was increased significantly in the group treated wth ST at the concentrations of 40 ng/ml, 60 ng/ml,100 ng/ml for 24 h,compared with the control group (P<0.01).The expression levels of p21^(WAFl)gene in both MGC803 and SGC7901 cells were markedly up-regulated after treatment with ST. CONCLUSION:ST can cause arrest of gastric cancer cells at G_2/M phase,which may be one of the mechanisms that inhibit cell proliferation and cause apoptosis in these cells. Effect of ST on cells at G_2/M phase may be attributed to the up-regulattion of p21^(WAFl) gene.

关 键 词：胃癌 细胞周期 细胞形态学 十字孢碱 作用机制 MGC803细胞 SGC7901细胞 RT-PCR 

分 类 号：R735.2[医药卫生—肿瘤]