Inhibition of β-ionone on SGC-7901 cell proliferation and upregulation of metalloproteinases-1 and -2 expression  

作　　者：Jia-RenLiu Bao-FengYang Bing-QingChen Yan-MeiYang Hong-WeiDong You-QiangSong 

机构地区：[1]PublicHealthCollege,HarbinMedicalUniversity,Harbin150001,HeilongjiangProvince,China [2]DepartmentofPharmacology,HarbinMedicalUniversity,Harbin150086,HeilongjiangProvince,China [3]DepartmentofBiochemistryandtheGenomeResearchCentre,TheUniversityofHongKong,HongKong,China

出　　处：《World Journal of Gastroenterology》2004年第2期167-171,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.30200229 and the Youth Foundation of Harbin Medical University,China

摘　　要：AIM:To observe the effect of β-ionone on the proliferation of human gastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase.METHODS:Using growth inhibition, Zymograms assays and reverse transcription-polymerase-chain reaction (RT-PCR),we examined cell growth rates,activities of matrix metalloproteinases-2 (MMP-2) and-9 (MMP-9),and expression of metalloproteinases-1 (TIMP-1) and-2 (TIMP-2) in SGC-7901 cells after the treatment with β-ionone for 24 h and 48h, respectively.RESULTS:β-ionone had an inhibitory effect on the growth of SGC-7901 cells.Eight days after the treatment with β-ionone at concentrations of 25, 50, 100 and 200μmol/L,the inhibition rates were 25.9%, 28.2%, 74.4% and 90.1%,respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89μmol/L.The effects of β-ionone on MMP-2 and MMP-9 activities in SGC-7901 cells were not observed. However,the levels of TIMP-1 and TIMP-2 transcripts were elevated in cells treated with β-ionone in a dose-dependent manner.CONCLUSION:β-ionone can inhibit the proliferation of SGC-7901 cells,upregulate the expression of TIMP-1 and TIMP-2 expression, and may influence metastasis of cancer.AIM:To observe the effect of β-ionone on the proliferation of human gastric adenocarcinoma cell line SGC-7901 and the inhibition of metalloproteinase. METHODS:Using growth inhibition,Zymograms assays and reverse transcription-polymerase-chain reaction (RT- PCR),we examined cell growth rates,activities of matrix metalloproteinases-2 (MMP-2) and -9 (MMP-9),and expression of metalloproteinases-1 (TIMP-1) and -2 (TIMP-2) in SGC- 7901 cells after the treatment with β-ionone for 24 h and 48 h,respectively. RESULTS:β-ionone had an inhibitory effect on the growth of SGC-7901 cells.Eight days after the treatment with β- ionone at concentrations of 25,50,100 and 200 μmol/L, the inhibition rates were 25.9%,28.2%,74.4% and 90.1%, respectively.The ICso value of β-ionone for SGC-7901 cells was estimated to be 89 μmol/L.The effects of β-ionone on MMP-2 and MMP-9 activities in SGC-7901 cells were not observed.However,the levels of TIMP-1 and TIMP-2 transcripts were elevated in cells treated with β-ionone in a dose-dependent manner. CONCLUSION:β-ionone can inhibit the proliferation of SGC- 7901 cells,upregulate the expression of TIMP-1 and TIMP- 2 expression,and may influence metastasis of cancer.

关 键 词：β-紫罗酮 SGC-7901细胞 细胞增殖 基质金属蛋白酶 逆转录聚合酶链反应 肿瘤细胞 

分 类 号：R730.5[医药卫生—肿瘤]