锌缺乏对生长期大鼠免疫细胞凋亡的影响  

EFFECT OF ZINC DEFICIENCY ON THE APOPTOSIS OF IMMUNOCYTES IN THREE WEEKS OLD RATS

作　　者：单延春 盛晓阳[2] 洪昭毅[2] 

机构地区：[1]青岛市海慈医疗集团儿科 [2]上海第二医科大学附属新华医院儿保科,青岛266033

出　　处：《营养学报》2004年第2期94-97,共4页Acta Nutrimenta Sinica

摘　　要：目的: 观察饲料锌缺乏对生长期大鼠免疫细胞凋亡的影响,并探讨其分子机制。方法: 通过喂食锌含量不同的饲料,构建生长期缺锌大鼠模型;TUNEL方法原位检测胸腺、脾脏组织淋巴细胞凋亡,半定量RT-PCR方法检测一对凋亡调控基因bcl-2/bax mRNA的表达。结果: 与锌充足组(ZA)和配饲组(PF)相比,缺锌组(ZD)大鼠胸腺、脾脏淋巴细胞凋亡增多,促凋亡基因bax mRNA表达增高,补锌后上述改变可得到恢复。结论: 饲料锌缺乏导致发育中和成熟的淋巴细胞凋亡增多,凋亡调控基因bcl-2/bax表达失衡是重要机制之一。Objective: To observe the effects of dietary zinc deficiency on the apoptosis of immunocytes in 3 weeks old rats and study the underlying mechanism. Methods: A zinc-deficient growing rat model was developed. Using TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thymocytes and spleen lymphocytes was checked. The expression of bcl-2, bax mRNA in thymus and spleen was detected by RT-PCR. Results: Compared with zinc adequate(ZA) and paired-fed(PF) groups, the percentage of apoptosis cells in thymus and spleen of zinc-deficient(ZD) rats was obviously higher, and the thymus and spleen of ZD rats had a higher expression of bax mRNA. Zinc supplementation could reverse these changes. Conclusion: Dietary zinc deficiency leads to the increase of apoptosis of lymphocytes in thymus and spleen. One important mechanism is that the thymus and spleen have higher expression of bax mRNA, which causes the decrease of bcl-2/bax ratio.

关 键 词：生长期 大鼠 免疫细胞 细胞凋亡 锌元素 TUNEL法 皮质激素 γ-射线 

分 类 号：R363[医药卫生—病理学]