一株鸭乙型肝炎病毒DHBV全基因的克隆和序列分析  

Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus

作　　者：梁蔚芳[1] 刘志华[1] 何海棠[1] 骆抗先[1] 

机构地区：[1]第一军医大学南方医院感染内科,广东广州510515

出　　处：《第一军医大学学报》2004年第4期404-407,共4页Journal of First Military Medical University

基　　金：军队"十五"重点课题(01Z046)~~

摘　　要：目的从本地樱桃谷鸭血清中克隆鸭乙型肝炎病毒(DHBV)DNA,并进行序列分析.方法用PCR扩增DHBV全基因,连接至T载体上,挑选克隆进行测序分析,与GenBank中16株DHBV全基因组进行同源性比较,并进行分子进化树分析.结果24-18与16株DHBV基因组比较,核苷酸同源性介于89.4%99.3%之间,各开放阅读框氨基酸同源性比较显示差异显著地方位于P区.结论24-18属于DHBV西方基因型中的一个亚型.Objective To clone and analyze the genome of a duck hepatitis B virus (DHBV) strain isolated from the ducks found in the local area. Methods The complete genome of DHBV was amplified by PCR prior to cloning into T vector and sequencing, with also homology and phylogenetic analyses. Result Genome comparison of an isolated DHBV strain and 16 DHBV strains in GenBank revealed a homology of 89.4% to 99.3% at the nucleotide level, and the amino acid identity of the 3 open reading frames showed that the P region harbored more obvious variations than the other regions. Conclusion The isolated DHBV strain might belong to a subtype of the virus found in the western countries.

关 键 词：鸭乙型肝炎病毒 DHBV全基因 克隆 序列分析 

分 类 号：R512.6[医药卫生—内科学] Q78[医药卫生—临床医学]