锌(Ⅱ)没食子酸的配位行为及其与DNA的相互作用  

Study on the Complexes of Gallic acid-Zn(Ⅱ) and the Interaction with DNA

作　　者：张连茹[1] 陈喀林[2] 林路[1] 杨洗[3] 邹国林[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072 [2]赤峰学院,内蒙古赤峰024001 [3]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2004年第2期234-238,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30370366)

摘　　要：用紫外光谱,荧光光谱以及高效液相色谱法,研究了没食子酸(GA)、Zn2+在接近生理条件下的配位行为及其与DNA的作用.探讨了溶液的pH值对配位行为的影响.计算了GA和Zn2+在溶液中的配位数,推测了二元配合物与DNA的作用方式,以及DNA对GA Zn2+体系的荧光淬灭常数.结果表明GA单独存在时,几乎不与DNA发生作用.当GA与Zn2+按1∶1形成配合物后,可以与DNA发生作用,三元复合物的形成,使体系的吸收光谱红移,荧光强度降低,HPLC的保留时间改变.GA在Zn2+介导下与DNA发生了较强的相互作用.The interaction of gallic acid-Zn(Ⅱ) complex with DNA in solution was studied. The result showed that DNA could bind to calf thymus DNA in the presence of Zn ions, which have been confirmed by absorption, fluorescence spectra and HPLC. The increase of peak intensity and red shift of gallic acid in the presence of Zn^(2+) showed that GA-Zn^(2+) binary complex was formed. Much more spectra data showed that ternary complex of GA-Zn^(2+)-DNA was formed, through quenching the fluorescence of GA-Zn^(2+), (using) Stern Volmer equation to calculate the quenching constant Kq, and the style of quenching. The results of HPLC also showed the existing of complexes, by comparing the different of retention time among that of GA, DNA, GA-Zn^(2+) and GA-Zn^(2+)-DNA. The complex number is calculated, by the method of Asmus iso-absorbency. Furthermore, the form of complexes also could be affected by the pH value of the solution.

关 键 词：锌离子 没食子酸 DNA 配合物 荧光淬灭 反应动力学 

分 类 号：O614.241[理学—无机化学]