人CEA信号肽-EK位点-HBD2融合基因的构建  

To construct fusion gene clone vector:pBluescript SK-CEA signal peptide-EK cutting peptide gene-HBD2

作　　者：杜剑平[1] 张青[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥全军肿瘤中心,重庆40003

出　　处：《安徽医科大学学报》2004年第2期87-89,共3页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金资助课题 (编号 :3 0 170 40 4)

摘　　要：目的　构建人CEA信号肽 EK激酶 HBD2抗菌肽融合基因。方法　PCR方法将CEA信号肽、EK激酶识别位点序列、上下游酶切位点及保护性碱基克隆在一个片段上 ,酶切后 ,T4DNA连接酶将此片段定向连接在含HBD2基因片段的 pBluescriptSK质粒上 ,构建成人CEA信号肽 EK激酶 HBD2抗菌肽融合基因 pBluescriptSK载体。 结果　融合基因经琼脂糖电泳、测序正确。结论　成功构建了人CEA信号肽 EK激酶 HBD2抗菌肽融合基因 pBluescriptSK克隆载体。Objective To construct fusion gene clone vector:pBluescript SK-CEA signal peptide-EK cutting peptide gene-HBD2 full length cDNA. Methods PCR reaction amplifies fusion gene sequence:CEA signal peptide-EK cutting peptide gene and up-downstream restriction site and two extra bases;after that the fusion gene sequence and pBluescript SK-HBD2 was cut by restriction enzymes;then the fusion gene inserted upstream of HBD2 in MCS of pBluescript SK-HBD2 by T4 ligase. Results The electrophoresis and sequencing were done,the fusion gene was between 250 bp to 500 bp,and sequencing proved it was target gene. Conclusion The fusion gene clone vector is constructed successfully.

关 键 词：人CEA信号肽-EK位点-HBD2融合基因 构建 遗传学 基因表达 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R394[医药卫生—基础医学]