dNCPs、TGF-β1及联合应用对人牙髓成纤维细胞增殖、ALP及矿化的影响  

The effects of dentin non-collagen proteins and transforming growth factor-β1 on the proliferation, ALP activity and mineralization of human dental pulp cells in vitro

作　　者：聂鑫[1] 金岩[1] 张勇杰[1] 赵守亮[1] Smith AJ 秦瑞峰[1] 

机构地区：[1]西安第四军医大学口腔医学院,710032 [2]英国伯明翰大学口腔病理科

出　　处：《实用口腔医学杂志》2004年第2期178-181,共4页Journal of Practical Stomatology

摘　　要：目的 :观察牙本质非胶原蛋白 (dentinnon collageproteins ;dNCPs)、转化生长因子 (transforminggrowthfactor ;TGF β1)及联合应用对人牙髓成纤维细胞生物学特性的影响。 方法 :利用混合酶消化法成功培养了人牙髓成纤维细胞 ,角蛋白及波形丝蛋白鉴定其来源。在细胞中分别加入牙本质粉、dNCPs、TGF β1及其复合物 ,通过MTT、ALPase和Von Kossa染色检测其作用。 结果 :dNCPs和TGF β1可显著增加细胞的增殖及ALPase分泌 ,细胞可聚集成团形成矿化结节。 结论 :dNCPs和TGF β1可改变人牙髓成纤维细胞的生物学活性 ,但两者间无相互促进作用。Objective: To study the effect of dentin non-collagen proteins (dNCPs) and transforming growth factor-β1 (TGF-β1) on the biological characteristic of human dental pulp (HDP) cells, Methods: HDP cells were primarily cultured with enzyme-digest technic and identified with immunohistochemical staining of keratins and waveform fibroins. The effects of dental powder, dNCPs, TGF-β1 or the combination of dental powder, dNCPs and TGF-β1 on human dental pulp(HDP) cells were studied with MTT assay, ALPase immunoassay and Von-Kossa staining. Results: dNCPs and TGF-β1 used singly or in combination increased the cell proliferation (P<0.05), ALPase activity(P<0.05) and the formation of mineralized nodule. Conclusion: dNCPs and TGF-β1 may stimulate the proliferation differentiation of HDP cells.

关 键 词：dNCPs TG-β1 联合应用 牙髓成纤维细胞增殖 ALP 矿化 牙本质非胶原蛋白 转化生长因子 

分 类 号：R780.2[医药卫生—口腔医学]