内皮抑素联合血管抑素诱导白血病细胞凋亡  

The effect of both rhES and hAS combined with apoptosis of leukemia cells

作　　者：赵怀顺[1] 王荷[2] 

机构地区：[1]兰州医学院医学实验中心,730000 [2]兰州医学院药学院药剂室

出　　处：《兰州医学院学报》2004年第1期4-7,共4页Journal of Lanzhou Medical College

摘　　要：目的　探讨重组人内皮抑制素因子 (rhES)联合人血管抑制素因子 (hAS)诱导白血病细胞凋亡的作用。方法　采用克隆 pCX的表达产物和用亲和层析及胰蛋白酶消化进行重组人内皮抑制素(rhES)和人血管抑制素 (hAS)的制备和纯化 ;以原代白血病细胞为靶细胞 ,应用MTT法、DNA琼脂糖电泳、流式细胞术检测白血病细胞凋亡。结果　内皮抑制素和血管抑制素联合对白血病细胞的生长、增殖有抑制作用 ,同时对白血病细胞具有诱导细胞凋亡作用 ,与单独相比有差异 (P <0 .0 1 )。结论　联合应用两种血管形成抑制因子对白血病细胞具有阻止细胞生长。Objective To investigate the apoptosis induction effect of recombined human endostatin factor(rhES) combined with and human agiostatin (hAS) on Leukemia cells.Methods The preparation and purification of rhES and hAS were processed via cloned with pCX expression, purified by affinity chromatography and digested by elastase. Culture leukemia marrow cells.was observed by use of MTT assay Linght microscopy, DNA agarose electr ophoresis and flow cytometry (FCM) were Employed to study the apoptosis induction effect of rhES and hAS combined with on Leukemia cells. Results rhES and hAS combined with apparent inhibitory effect of growth and proliferation on leukemia cells; The content were suppressed by rhES/hAS combined with apoptosis induction effects on Leukmia cells. compare with single factor group and medication group have significant ditference( P <0.01). Conclusion To applicate Angiogenesis growth inhibitor combination with synergistic action inhibiting cell growth. Proliferation and inducing cell apoptosis and have dowble effect inhibiting to secret of tumor and express receptor in cell surface.

关 键 词：内皮抑素 血管抑素 白血病 细胞凋亡 流式细胞术 MTT法 DNA琼脂糖电泳 血管形成抑制因子 

分 类 号：R733.7[医药卫生—肿瘤]