一种改进的齿突蟾动物肌肉线粒体DNA提取方法  

AN IMPROVED METHOD FOR ISOLATION OF ANIMAL MUSCLE MITOCHONDRIAL DNA

作　　者：莫邦辉[1] 袁水全 郑渝池[1] 郭骁才[1] 曾晓茂[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]成都市第一农业科学研究所,成都6110072

出　　处：《应用与环境生物学报》2004年第2期249-250,共2页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金30070090(NSFC;No.30070090);中国科学院西南基地创新工程资助~~

摘　　要：以1号冷冻保存(-20℃)的齿突蟾标本为材料,建立一种动物肌肉线粒体DNA的快捷提取方法.取10 g冷冻保存的肌肉组织,在研钵中剪碎,液氮研磨成粉;然后加SE缓冲液混匀,转入10 mL离心管静置10 min;上清液转入2 mL的Eppendorf管,1 000×g离心10 min,取上清液,再12 000×g离心15 min,沉淀即为线粒体;最后用SDS碱裂解法分离得到mtDNA.In this paper a mitochondrial DNA (mtDNA) extraction method using single specimen of Scutiger ruginosus frozen at -20℃ was establishment. 10 gram of the tissue was cut into small particles with scissors, and ground to fine powder with a mortal and pestle in liquid nitrogen. Then SE buffer was added into mortal, mixed well and transferred to 10 mL centrifuge tube. The tube was placed stably for 10 min. The supernatant was carefully transferred to 2 mL Eppendorf tube, and centrifuged at 1 000 ×g for 10 min. Again, the supernatant was carefully transferred into a new Eppendorf tube and centrifuged at 12 000 ×g for 15 min. The pellet was mitochondria. At last mtDNA was extracted by SDS alkaline lysis from the mitochondria. Fig 1, Ref 3

关 键 词：齿突蟾 动物肌肉 线粒体DNA 提取方法 MTDNA 

分 类 号：Q503[生物学—生物化学]