中药红花与产纤溶酶菌株相互影响的研究  

Study on Interaction of Carthamus Tinctorius L. and Strain Producing Fibrinnolytic Enzyme

作　　者：冯志华[1] 孙启玲[1] 米坤[1] 罗强[2] 魏琪[1] 高婷[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064 [2]乐山师范学院环境与生命科学系,四川乐山614004

出　　处：《四川大学学报（自然科学版）》2004年第2期395-398,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省中医药管理局基金项目(200214)

摘　　要：作者采用生长曲线法衡量不同浓度红花对产纤溶酶菌株C2 13的生长影响;纤维蛋白平板法测量纤溶酶活性;邻二氮菲 Fe2+氧化法测量羟自由基清除率;乙酸酐直接测定法测量总胆固醇;HPLC法分析发酵产物.结果表明:产纤溶酶菌株C2 13能显著提高红花降血清总胆固醇的功效以及抗羟自由基氧化的能力;一定浓度红花的加入对C2 13的生长和纤溶酶活性有明显促进作用.HPLC分析还观察到红花经发酵炮制后,其成分发生了改变.说明中药红花与产纤溶酶菌株C2 13可相辅相成,互相促进.The interaction of Carthamus tinctorius L(C.T.L.) and C2-13 were studied. Growth of C2-13adding different concentrations of C.T.L.was examined by growth curve model. The activity of fibrinnolytic enzyme was measured by fibrinplate method. Eliminating ratio of hydroxyl radical is determined using 1,10-Phenanthroline-Fe^(2+)oxidative assay. Total cholesterol is examined by direct acetic anhydride determination. Products of fermentation are analyzed by HPLC method.The resultsshowed that C2-13 could raise greatly the effect of promoting blood circulation and the ability of anti-oxidation of hydroxyl radical. Growth of the strain and the activity of fibrinnolytic enzyme producing by it can be promoted greatly by adding a certain concentration of C.T.L.Using HPLC assay, it was observed that the fermentation and processing of C.T.L by the strain could improve the effective components of C.T.L.The C.T.L.and C2-13 could supplement and improve each other.

关 键 词：红花 地衣芽孢杆菌 发酵 炮制 纤溶酶 

分 类 号：Q946[生物学—植物学] Q935