负载肝癌排斥抗原肽的树突状细胞瘤苗活化T细胞的应用  

Application of activated T lymphocytes induced by hepatocellular carcinoma rejection antigenic peptide-pulsed DCs in vivo and in vitro

作　　者：曲萍[1] 曹云新[2] 隋延仿[1] 张立红[3] 张秀敏[1] 

机构地区：[1]第四军医大学基础医学部病理学教研室,西安710032 [2]第四军医大学免疫学教研室,西安710032 [3]第四军医大学生物化学教研室,西安710032

出　　处：《中国药理学通报》2004年第4期389-392,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金重点项目资助课题 ;No 3 983 0 42 0

摘　　要：目的　探讨负载肝癌排斥抗原肽SLIVHLNEV(C met突变肽 ) [1 ] 的树突状细胞瘤苗活化的T细胞在体外及裸鼠肝癌模型体内诱导的特异性抗肿瘤免疫。方法　用肝癌排斥抗原肽SLIVHLNEV致敏从脾脏中分离培养的树突状细胞 ,再与同源T淋巴细胞混合培养。乳酸脱氢酶 (LDH)释放法检测细胞毒作用。同时建立荷人肝癌细胞系HHCC的裸鼠移植瘤模型 ,观察DC瘤苗活化的T细胞预防移植瘤发生和抑制移植瘤生长的作用。结果　在体外实验中 ,DC瘤苗诱导的CTLs能够特异性杀伤HHCC肝癌细胞。在裸鼠模型中 ,CTLs不但能预防裸鼠移植瘤的发生 ,而且抑制裸鼠移植瘤生长。AIM To study if the activated T lymphocytes induced by hepatocellular carcinoma (HCC) rejection antigenic peptide SLIVHLNEV (mutant peptide of C met) pulsed dendritic cells (DCs) can provide protection against tumor challenge in nude mice HCC model and in vitro . METHODS SLIVHLNEV was eluted from HCC cell line HHCC by mild acid buffer and was identified by the mass spectrometry technique. SLIVHLNEV pulsed DCs isolated from HLA A2+ ruptured spleen were co cutured with isogeneic T lymphocytes. Cytotoxicity was tested using lactate dehydrogenase (LDH) release assay. The induced cytotoxic T lymphocytes (CTLs) were implanted into nude mice in order to protect them against transplanted HHCC tumor cells. The inhibition effect of CTLs on the growth of implanted tumor in nude mice was also observed. RESULTS The CTLs induced by SLIVHLNEV pulsed DCs had unique ability to kill HHCC tumor cells in vitro. They also protected the nude mice against the further challenge of HHCC tumor cells and inhibited the growth of implanted tumor. CONCLUSION DC based HCC rejection antigenic peptide SLIVHLNEV vaccine can induce powerful immune reaction both in nude mice HCC model and in vitro.

关 键 词：肿瘤排斥抗原肽 癌 肝细胞 树突细胞 瘤苗 

分 类 号：R-332[医药卫生] R341.6