蒺藜皂苷对缺血再灌注损伤心肌细胞的保护作用  

Protective effect of GSTT on myocardium injuried by ischemia reperfusion

作　　者：侯俊英[1] 王秀华[1] 李红[1] 杨世杰[1] 

机构地区：[1]吉林大学基础医学院药理学教研室,长春130021

出　　处：《中国药理学通报》2004年第4期418-420,共3页Chinese Pharmacological Bulletin

摘　　要：目的　研究蒺藜皂苷 (GSTT)对缺血再灌注损伤心肌细胞的保护作用。方法　通过模拟缺血再灌注模型 ,研究GSTT对再灌注后LDH、CK漏出量 ,MDA含量 ,SOD活性 ,以及TNF α和IL 6分泌量 ;用浓度为 1 6 2 5ng·L-1 TNF α损伤心肌细胞 ,研究GSTT对培养液中NO生成量的影响 ,并用MTT法检测GSTT对TNF α损伤心肌细胞线粒体酶的保护作用。结果　GSTT可抑制再灌注后LDH、CK漏出量 ,减少MDA含量 ,升高SOD活性 ,抑制TNF α和IL 6的分泌量 ;减少TNF α引起NO生成量 ,升高OD490 吸光度值。结论　GSTT可减轻缺血再灌注对心肌细胞的损伤程度 ,对心肌细胞具有直接的保护作用。AIM To investigate the protective effect of GSTT on myocardial injury induced by ischemia reperfusion. METHODS The content of lactic dehydrogenase(LDH),creatine kinase(CK),methylenedioxyamphetamine(MDA),superoxide dismutase(SOD),tumor necrosis factor alpha(TNF α) and interleukine 6(IL 6) were determined in medium after reperfusion by simulating model of ischemia reperfusion; The content of nitric oxide(NO) was determined in medium by TNF α injury myocardium,and mitochondrial activity was determined by MTT after TNF α stimulated. RESULTS Compared with the model control,in the GSTT, the content of LDH,CK,MDA,TNF α and IL 6 in medium decreased and activity of SOD increased after reperfusoin,the level of NO in medium decreased and OD 490 increased after TNF α injuried. CONCLUSION It is suggested that GSTT can mitigate injury of ischemia reperfusion on myocardium, and has a direct protective effect on injuried myocardium.

关 键 词：蒺藜皂苷 心肌细胞培养 细胞因子 线粒体酶活性 

分 类 号：R-332[医药卫生] R282.71