苦参素诱导卵巢癌HO8910细胞凋亡的初步研究  

Studies on oxymatrine induces apoptosis in ovarian cancer cell lines HO8910

作　　者：高洪泉[1] 王英[1] 杨春壮[1] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中医药学报》2004年第2期52-54,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省自然科学基金资助项目 (D0 2 58) ;黑龙江省卫生厅科技处基金资助项目 (2 0 0 2 - 0 98)

摘　　要：目的 :观察苦参素对卵巢癌细胞株HO8910的抑制增殖效应及凋亡诱导作用。方法 :用苦参素处理HO8910细胞 ,采用MTT法检测药物对细胞的抑制作用 ,倒置显微镜观察细胞形态学改变 ;琼脂糖凝胶电泳观察DNA降解 ,以及应用流式细胞仪观察细胞凋亡过程中细胞周期的变化。结果 :在苦参素作用下 ,HO8910细胞呈凋亡改变 ,DNA琼脂糖凝胶电泳呈典型的凋亡特征。细胞凋亡的同时 ,细胞周期发生特定的改变。结论 :苦参素能抑制卵巢癌细胞增殖 ,诱导卵巢癌细胞凋亡。Objective:The current study was designed to investigate whether oxymatrine can induces apoptosis in ovarian cancer cell.Methods:Cell proliferation was assessed by MTT assays.Morphological changes of apoptosis were observed with invert microscope.DNA ladder and cell cycle were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry(FCM),respectively.Results:Under the influence of oxymatrine,HO8910 cell exhibited changes of apoptosis both in morphology and in the characteristics of electrophoresis.Meanwhile the cell cycle also showed special change.Conclusion:Oxymatrine can inhibit cell proliferation,oxymatrine can induces apoptosis in ovarian cancer cell. Author's address:Mudanjiang Medical Collage,Mudanjiang 157011,China

关 键 词：苦参素 卵巢癌 HO8910细胞 细胞凋亡 细胞增殖 细胞形态学 琼脂糖凝胶电泳 流式细胞仪 

分 类 号：R737.31[医药卫生—肿瘤] R965[医药卫生—临床医学]