邻苯二甲酸二丁酯对大鼠精子运动能力及氧化应激的影响  

Effects of Di-butyl Phthalate on Sperm Motility and Oxidative Stress in Rats

作　　者：王玉邦 宋玲[1] 陈建锋[1] 何俊[1] 刘茹[1] 朱正平[1] 王心如[1] 

机构地区：[1]南京医科大学应用毒理研究所,江苏南京210029

出　　处：《中华男科学杂志》2004年第4期253-256,共4页National Journal of Andrology

基　　金：国家基础研究重大项目前期研究专项 (国科基字 [2 0 0 1] 5 0号 )

摘　　要：目的 :探讨邻苯二甲酸二丁酯 (DBP)对大鼠睾丸抗氧化系统和精子运动能力的影响。　方法 :将 6周龄雄性SD大鼠随机分成 0 (对照组 )、2 5 0、5 0 0、10 0 0mg/(kg·d) 4组 ,每组 8只 ,灌胃给予DBP ,共染毒 4周。用计算机辅助精子分析系统 (CASA)观察附睾尾部精子运动速度参数 :曲线运动速度 (VCL)、直线运动速度 (VSL)、平均路径速度 (VAP)、鞭打频率 (BCF)和精子运动方式参数 :精子头侧摆幅度 (ALH)、直线性 (LIN)、平均移动角度 (MAD)、前向性 (STR)的变化。用分光光度法测定血清和睾丸匀浆中超氧化物歧化酶 (SOD)活力和丙二醛 (MDA)含量 ,同时观察每日体重增加量及肝、睾丸、附睾等脏器系数的变化。　结果 :染毒后肝脏器系数显著上升 ,各剂量组与对照组相比差异均有显著性 (P <0 .0 1) ;睾丸脏器系数下降 ,10 0 0mg/(kg·d)组与对照组相比P <0 .0 1;VCL、ALH随染毒剂量的增加呈下降趋势 ,10 0 0mg/(kg·d)组与对照组相比差异均有显著性 (P <0 .0 5 ) ;睾丸匀浆SOD活力降低 ,10 0 0mg/(kg·d)组与对照组相比差异有显著性 (P <0 .0 5 ) ;其他指标的改变差异无显著性。　结论 :DBP可引起大鼠睾丸精子运动能力和抗氧化能力的下降。睾丸是DBP毒作用的主要靶器官之一。Objective: To evaluate the effects of di-butyl phthalate(DBP) on the sperm motility and oxidative stress in rats. Methods: Healthy 6-week-old male Sprague Dawlay rats were randomly divided into 4 groups with 8 in each group. DBP dissolved in peanut oil was administered by gavage at dosage of 0,250,500, 1 000 mg/(kg·d) . After 4-week DBP exposure, the animals were killed and the organs were selected and weighed. The sperm VCL, VSL, VAP, BCF, ALH, LIN, MAD and STR in the cauda epididymis were assessed by computer assisted sperm analysis (CASA), and the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in serum and testis homogenate were measured simultaneously. The increase of body weight per day and the organ body weight ratio changes of the liver, testes and epididymides were also observed. Results: The liver organ body weight ratios of the treated groups were higher than those of the control (P< 0.01 ), while the testis organ body weight ratios were lower at dosage of 1 000 mg/(kg·d) DBP. Compared with the control group, the parameters of rat sperm VCL and ALH declined significantly at dosage of 1 000 mg/(kg·d) DBP. In addition, DBP showed inhibiting effect on SOD activities in the testis, and it was significant in the highest exposure group compared with the control (P< 0.05 ). However, there were no differences in serum SOD activities between the treated groups and the control. Conclusion: DBP exposure may affect the sperm motility and the anti-oxidative systems. The testis is a vital target organ influenced by DBP.

关 键 词：邻苯二甲酸二丁酯 精子 计算机辅助精子分析 氧化应激 大鼠 

分 类 号：R363[医药卫生—病理学]