马铃薯叶片高效再生体系的建立  

Establishment of efficient regeneration system from leaf explants of potato

作　　者：李娟[1] 程智慧[1] 张国裕[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2004年第4期610-614,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中德政府间合作项目(GH-01/04)

摘　　要：以4个马铃薯栽培品种为试材,进行了叶片离体再生研究,结果表明:东农303、鄂1号叶片愈伤组织诱导的最佳培养基为MS+6-BA2.5mg·L-1+NAA0.2mg·L-1;费乌瑞它为MS+6-BA2.0mg·L-1+NAA0.1mg·L-1;夏波帝为MS+6-BA2.0mg·L-1+NAA0.2mg·L-1,愈伤组织的诱导率均可达100%.诱导不定芽分化的最佳培养基分别是:费乌瑞它、鄂1号为MS+6-BA2.5mg·L-1+GA35.0mg·L-1;东农303为MS+6-BA1.0mg·L-1+IAA0.1mg·L-1+GA32.5mg·L-1;夏波帝为MS+6-BA2.5mg·L-1+IAA0.5mg·L-1+GA32.5mg·L-1,其不定芽分化率分别达94.3%、100%、100%和90%.The regeneration system of four cultivated potatoes in vitro was studied with leaves as explants,and the results showed that the optimal medium for callus induction from leaf discs was MS+6-BA 2.5 mg·L^(-1)+NAA 0.2 mg·L^(-1) for “Dongnong 303” and “E No.1”,MS+6-BA 2.0 mg·L^(-1)+NAA 0.1 mg·L^(-1) for “Favorite” and MS+6-BA 2.0 mg·L^(-1)+NAA 0.2 mg·L^(-1) for “Sharpdi”.The optimal medium for adventitious bud differentiation from leaf-derived calluses was found to be MS+6-BA 2.5 mg·L^(-1)+GA_3 5.0 mg·L^(-1) for “Favorite” and “E No.1,MS+6-BA 1.0 mg·L^(-1)+IAA 0.1 mg·L^(-1)+GA_3 2.5 mg·L^(-1) for “Dongnong 303”,MS+6-BA 2.5 mg·L^(-1)+IAA 0.5 mg·L^(-1)+GA_3 2.5 mg·L^(-1) for “Sharpdi”.Adventitious bud differentiation rate of “Dongnong 303”,“Sharpdi”,“Favorite” and “E No.1” reached 94.3%,100%,100% and 90% respectively.

关 键 词：马铃薯 叶片 愈伤组织 植株再生 

分 类 号：Q943.1[生物学—植物学]