应用组织芯片技术检测核仁组成区相关蛋白对乳腺导管不典型增生和导管原位癌的诊断意义  

Significance of the diagnosis of AgNOR analysis in atypical ductal hyperplasia and ductal carcinomain situ of the breast by the application of tissue mi croarray technology

作　　者：刘晖[1] 张长淮[2] 

机构地区：[1]首都医科大学附属北京天坛医院病理科,北京100050 [2]首都医科大学附属北京友谊医院病理科,北京100050

出　　处：《临床和实验医学杂志》2004年第1期3-5,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的　探讨核仁组成区相关蛋白 (AgNOR)对乳腺导管不典型增生 (ADH)和导管原位癌 (DCIS)的诊断意义。方法　应用组织芯片技术对 2 0例乳腺不典型增生和 2 0例乳腺导管原位癌的AgNOR进行图象定量分析 ,获得四个参数 ,包括 :每个细胞AgNOR颗粒平均个数、AgNOR颗粒总面积、单个颗粒AgNOR平均面积、AgNOR面积 核面积。结果　每个细胞AgNOR颗粒平均个数、AgNOR颗粒总面积两个参数ADH和DCIS的差异有统计学意义 (P =0 0 0 0 ,P =0 0 0 1) ,DCIS明显高于ADH。这两个参数ADH和低度DCIS的差异也有统计学意义 (P =0 0 0 0 ,P =0 0 0 3 )。结论　AgNOR图象定量分析结果可以作为辅助诊断ADH和DCIS的指标。组织芯片技术有良好的应用价值和广阔的应用前景。Objective To explore the diagn ostic significance of argyrophilic nucleolar organizer regions ( AgNORs) in atyp ical ductal hyperplasia (ADH) and ductal carcinoma in situ (DCIS) of the breast . Methods Image analysis of AgNOR i n tissue microarray sections from biopsies of 20 breast ADH and 20 breast DCIS w as by the application of tissue microarray technology.Four parameters were acqu ire d,including the number of AgNORs per nucleus,total area of AgNORs,mean area o f a single AgNOR and the ratio AgNOR sum area/nuclear area. Results AgNOR number and total AgNOR are a increased from ADH to DCIS,and the differences between A DH and DCIS(low grade) were statistically significant.The other tw o parameters also increased from ADH to DCIS,but the differences were not stati stically significant. Conclusion AgNOR may be regarded as an assistant index of the diagnosis of ADH and DCIS.Tissue micr oarray technology has important practical significance and broad application pro spect in pathology.

关 键 词：乳腺肿瘤 核仁组成区相关蛋白 不典型增生 原位癌 图像分析 组织芯片 

分 类 号：R737.9[医药卫生—肿瘤]