重组腺病毒IκBαM在血管内皮细胞ECV-304中的表达及对NF-κB活性的抑制  

Expression of recombinant adenovirus IκBαM in vascular endothelial cells ECV-304 and its inhibitive effect to the activity of NF-κB

作　　者：肖彧君[1] 邓华聪[2] 黄爱龙[3] 

机构地区：[1]中国人民解放军总医院内分泌科,北京100853 [2]重庆医科大学附属第一医院内分泌科,重庆400010 [3]重庆医科大学肝炎研究所,重庆400010

出　　处：《中国急救医学》2004年第4期275-277,共3页Chinese Journal of Critical Care Medicine

基　　金：国家"十五"863资助项目 ( 2 0 0 1AA2 1712 1);国家自然科学基金资助项目( 3 0 0 70 2 97)

摘　　要：目的　检测重组腺病毒IκBαM(AdIκBαM)在血管内皮细胞ECV - 30 4中的表达及肿瘤坏死因子 (TNF -α)诱导下IκBαM的变化与对NF -κB活性的抑制作用。方法　用携有NF -κB抑制物IκBα突变体的AdIκBαM感染ECV - 30 4细胞 ,用West ernblot及EMSA法检测IκBαM的表达及NF -κB活性的变化情况。结果　AdIκBαM在ECV - 30 4细胞中表达且不被TNF -α诱导降解 ,感染IκBαM的ECV - 30 4 /IκBαM细胞在TNF -α刺激下NF -κB无激活 ,而未感染IκBαM的ECV - 30 4细胞经TNF -α刺激后可有NF -κB的过度活化。结论　AdIκBαM能高效扩增并有效感染ECV - 30 4血管内皮细胞 ,且不会被TNF -α诱导而降解 ,能有效抑制血管内皮细胞NF -κB的过度活化 ,抑制NF -κB活性可能保护血管内皮细胞免于TNFObjective To investigate the expression of recombinant adenovirus IκBαM in vascular endothelial cells ECV-304 and its inhibiting effect to NF-κB Methods Recombinant adenovirus mediated NF-κB supper-repressor IκBαM with mutant IκBα was constructed Western blot and electrophoretic mobility shift assay (EMSA) were applied in this study Results IκBαM could be expressed stably and efficiently in ECV-304 cells and would not degrade by induction of TNF-α EMSA demonstrated that the infected cells showed no activation of NF-κB before and after the treatment of TNF-α,the cells of uninfected and infected with AdIκBα appeared excessive activation of NF-κB Conclusion AdIκBαM can effectually inhibit the excessive activation of NF-κB in ECV-304 cells Inhibition of NF-κB activation can protect vascular endothelial cells from the injury of TNF-α

关 键 词：核转录因子-ΚB 肿瘤坏死因子 血管内皮细胞 

分 类 号：R363[医药卫生—病理学]