EBV-LMP2多肽所激活的特异性CTL对鼻咽癌细胞杀伤活性的研究  

A Study on Killing Effect of Cytotoxic T Cell Activated by LMP2 Peptides in Nasopharyngeal Carcinoma Cells

作　　者：杜海军[1] 周玲[1] 左建民[1] 王琦[1] 李红霞[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《肿瘤学杂志》2004年第2期92-94,共3页Journal of Chinese Oncology

基　　金：国家自然科学基金资助(基金编号为3020520);国家"973"资助项目(G1998051201);国家"863"资助项目(2001AA217091)

摘　　要：[目的]研究EB病毒编码的LMP2多肽片段所激活的肿瘤特异性CTL对鼻咽癌细胞的杀伤作用,探讨利用LMP2多肽对鼻咽癌进行免疫治疗。[方法]通过细胞免疫方法鉴定鼻咽癌细胞(CNE2)中的LMP2蛋白是否表达;外周血分离树突状细胞(DC)体外培养,以LMP2多肽片段负载DC激活产生特异性CTL;通过流式细胞仪、MTT法检测特异性CTL对CNE2细胞杀伤活性。[结果]在CNE2中表达LMP2蛋白,LMP2多肽所激活的肿瘤特异性CTL对鼻咽癌细胞具有杀伤活性。[结论]LMP2多肽片段所激活的肿瘤特异性CTL在体外对鼻咽癌细胞具有杀伤作用。To explore the possibility of the immunotherapy with specific cytotoxic T cell(CTL)acti-vated by LMP2peptides for NPC.Latent membrane protein2(LMP2)encoded by Epstein-Barr virus was identified by the means of cytoimmunity.Dendritic cells(DC)were separated from peripheral blood cells and cultured.The killing activity was demonstrated by flow cytometry.Tumorspecific CTL responses were ac-tivited by LMP2peptides presented by DC.Killing effect was detected by the way of MTT and flowcytometer.Expression of LMP2was found in CNE2.Specific CTL activated by LMP2peptides,which revealed killing activity to nasophargngeal carcinoma cells in vitro culture.[Conclusion]CTL activated by LMP2pep-tides has killing effect to nasopharyngeal carcinoma cells in vitro.

关 键 词：LMP2多肽 免疫疗法 树突状细胞 肿瘤特异性CTL 

分 类 号：R73-362[医药卫生—肿瘤]