VEGF硫代反义寡核苷酸抑制U937细胞VEGF的表达  

Inhibiting Effect of Vascular Endothelial Growth Factor (VEGF) Antisense Oligodeoxynucleotides on VEGF Expression in U937 Cell

作　　者：童允洁[1] 张敏[1] 邹萍[1] 郭荣[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022

出　　处：《中国实验血液学杂志》2004年第2期151-153,共3页Journal of Experimental Hematology

摘　　要：为研究硫代磷酸化修饰的血管内皮生长因子反义脱氧寡核苷酸 (VEGF ASODN)对急性单核细胞白血病细胞系U937VEGF表达的影响 ,将终浓度分别为 10 ,2 0 ,30 μmol/L的VEGF ASODN和错义序列与U937细胞分别孵育 2 4 ,4 8,72小时 ,采用RT PCR检测VEGFmRNA的表达 ,Westernblot检测VEGF蛋白的表达。结果显示 ,VEGF ASODN对U937细胞的VEGF的表达有明显的抑制作用 ,与错义序列组和空白对照组相比有显著差异 (P<0 .0 5 ) ;错义序列组与空白对照组VEGF的表达无显著差异 (P >0 .0 5 )。结论 :VEGFASODN可下调白血病细胞株U937的VEGFmRNA和蛋白的表达水平。To study the effect of VEGF fully phosporothioated antisense oligodeoxynucleotide (VEGF ASODN) on VEGF expression in acute monocyte leukemic cell line U937 in vitro, U937 cells were incubated with VEGF ASODN at concentrations of 10, 20 and 30 μmol/L or scrambled sequence as compared with negative control. The expression of VEGF mRNA was measured by semi quantitative RT PCR. The expression of VEGF protein was measured by Western blot. The result showed that VEGF ASODN had obviously inhibitive effect on expression of VEGF in U937 cell, as compared with scrambled sequence and negative control (P<0.05). Scrambled sequence group had no significant difference compared with negative control group (P>0.05). It is concluded that the expressions of VEGF mRNA and protein in leukemic cell line U937 are down regulated by VEGF ASODN.

关 键 词：VEGF 反义脱氧寡核苷酸 U937细胞 

分 类 号：R733.7[医药卫生—肿瘤]