PIP mRNA在乳腺癌组织中的表达  

The Expression of Prolactin-inducible prote in (PIP) Gene in Breast Cancer

作　　者：刘宁[1] 张伟[2] 郝希山[1] 李强[1] 王洪平[2] 金玉生[2] 刘毅[2] 曲平[2] 李茉[2] 曹冬艳[2] 邵永孚[3] 

机构地区：[1]天津医科大学附属肿瘤医院腹科,天津市300060 [2]中国医学科学院中国协和医科大学肿瘤研究所生物检测中心 [3]中国医学科学院肿瘤医院腹外科

出　　处：《中国肿瘤临床》2004年第3期134-137,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:本研究探讨乳腺组织特异性基因PIP(prolactin-inducibleprotein)mRNA在乳腺癌组织中的表达及其作为检测乳腺癌微小转移指标的可能性。方法:利用RT-PCR检测PIPmRNA乳腺癌患者癌组织和相应癌旁正常乳腺组织44例,以及胃癌、大肠癌、食管癌、肺癌、卵巢癌标本各5例中的表达。分析PIPmRNA表达与乳腺癌临床病理特点之间的关系。结果:44例乳腺癌组织和相应癌旁组织中43例表达PIPmRNA,其中有69.8%(30/43)的癌组织相对正常组织低表达;而胃癌、大肠癌、食管癌均未表达。肺癌、卵巢癌各1例PIP阳性。在乳腺癌组织和其它癌组织中表达有显著性差异(P<0.01)。PIP表达与乳腺癌病理类型、临床分期、ER、PR状态不相关(P>0.05)。结论:PIPmRNA作为乳腺组织特异性标志物可能成为检测乳腺癌微小转移的指标。Objective: The gene prolactin-inducible protein (PIP/GCDPF15) is believed to originate from a limited set of tissues, including breast and has been applied as a clinical marker of human breast cancer metastasis. We examined PIP mRNA expression as a marker for the detection of carcinoma cells by reverse transcription polymerase chain reaction (RT-PCR). Methods: Total RNA, which was extracted from 44 primary breast carcinomas and corresponding normal tissues, and 25 other carcinomas (including gastric carcinoma, esophageal carcinoma, lung carcinoma, ovary carcinoma and carcinoma of the large intestine) was subjected to analysis of PIP mRNA expression by the RT-PCR. The relationships between clinicopathological characteristics of the patients and the results of RT-PCR was analyzed by statistics. Results: PIP mRNA expression was detected by RT-PCR in 43 of 44 breast carcinomas. In the analysis, PIP mRNA levels were decreased relative to normal breast tissue in 69.8% (30/43) of cases. there were 2 positive in 25 other cancers. There is obviously different between breast cancer and other cancers in the expression of the PIP gene (P<0.01). There is no relationship between the expression of the PIP gene and the stage and the statuses of ER and PR of the patients (P>0.05). Conclusion: Whether PIP mRNA can be a marker in the detection of micrometastasis of breast cancer depends on further study.

关 键 词：乳腺癌 转移 PIP 

分 类 号：R737.9[医药卫生—肿瘤] Q344.13[医药卫生—临床医学]