神经激肽-3受体在胰腺癌组织中表达的初步研究  

Study of the expression of neurokinin-3 receptor in pancreatic carcinoma

作　　者：石欣[1] 高乃荣[1] 郭庆明[2] 胡浩霖[1] 霍明东[1] 汤文浩[1] 

机构地区：[1]东南大学附属中大医院普外科,南京210009 [2]东南大学附属中大医院病理科,南京210009

出　　处：《江苏医药》2003年第7期496-498,共3页Jiangsu Medical Journal

基　　金：国家人事部留学回国人员科研启动基金 (No .7690 0 0 40 2 7)

摘　　要：目的　研究神经激肽 3受体 (NK 3R)在胰腺癌组织中的表达 ,并将结果结合临床资料分析 ,以期找出其中相关性。方法　正常胰腺组织取自 2 0个器官捐献者 ,男性 11例 ,女性 9例 ;33个胰腺癌标本取自胰腺癌根治术 ,男性 19例 ,女性 14例。应用Northernblot技术 ,检测正常胰腺、胰腺癌组织和 7株胰腺癌细胞中NK 3R的mRNA水平 ,应用Westernblot检测其蛋白的表达水平。结果　与正常胰腺相比 ,胰腺癌组织中NK 3RmRNA过度表达 ,其表达水平与临床分期和术后生存时间相关 (P <0 0 5 ) ,胰腺癌组织中NK 3R蛋白也过度表达。结论　胰腺癌组织中NK 3R的表达水平明显上调 ,扰乱了神经激肽的作用环节 ,从而促进胰腺癌细胞的生长。Objective To investigate the expression of neurokinin-3 receptor(NK-3R)in pancreatic cancer and evaluate the relationship between expression of this receptor and clinical aspect. Methods Pancreatic carcinoma tissues were obtained from 19 men and 14 women undergoing a pancreatoduodenectomy. Normal human pancreatic tissues were obtained from 20 patients(11 males/9 females) as organ donor.The expression of NK-3R mRNA was investigated using Northern blot in normal pancreas and pancreatic cancer tissues.The level of NK-3R mRNA determined by Northern blot in pancreatic cancer samples was analyzed as regards to the tumor stage,pathological grade and post-operative survival in order to investigate whether correlation exists.The protein level of NK-3R was investigated by Western blot.Results NK-3R mRNA and protein were overexpressed in pancreatic cancer tissue when compared with normal pancreas.Overexpression of NK-3R was related to advanced tumor stages( P< 0.05) and postoperative survival( P< 0.05),suggesting that NK-3R may play an important role in the progression of human pancreatic cancer.Conclusions NK-3R was overexpressed in pancreatic carcinoma,and overexpression of NK-3R mRNA disrupts the balance of neuropeptide loops and changes pathoclinical aspect of pancreatic carcinoma.

关 键 词：神经激肽-3受体 胰腺癌 癌组织 肿瘤转移 P物质 生物学效应 

分 类 号：R735.9[医药卫生—肿瘤]