肝细胞癌组织中TGF-β_1基因mRNA和蛋白表达的研究  

Studies on expression of TGF-β_1 mRNA and protein in patients with hepatocellular carcinoma

作　　者：季国忠[1] 赵志泉[2] 缪林[1] 刘政[1] 张平[1] 杨春[1] 黄坚[3] 

机构地区：[1]南京医科大学第二附属医院,210011 [2]江苏省人民医院 [3]江苏省启东肝癌研究所

出　　处：《江苏医药》2003年第7期485-487,I002,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金资助课题 (BK2 0 0 1168)

摘　　要：目的　从转录和翻译两个水平探讨转化生长因子 β1(TGF β1)在肝细胞癌 (HCC)中的表达。方法　用免疫组化技术 ,分别检测 36例肝癌组织和癌旁肝组织中TGF β1及转化生长因子 β1Ⅱ型受体 (TβRⅡ )蛋白的表达 ;用原位杂交方法检测 2 6例肝癌组织和癌旁肝组织中TGF β1mRNA的表达 ,并将TGF β1蛋白与TGF β1mRNA ,TGF β1mRNA与TβRⅡ蛋白进行比较。 结果　 (1)肝癌组织中TGF β1mRNA、TGF β1蛋白表达均明显高于癌旁组织 (P <0 0 1) ;肝癌组织TβRⅡ蛋白表达明显低于癌旁组织 (P <0 0 1)。 (2 )肝癌组织中TGF β1mRNA与TGF β1蛋白呈正相关 ,TGF β1mRNA与TβRⅡ蛋白表达呈负相关。 结论　肝癌组织中TGF β1基因的变化发生在多个水平 ;TGF β1在肝癌发生中发挥重要作用 ;TGF β1抑制作用的减弱主要是由于TβRⅡ蛋白表达减弱引起。本研究为肝癌发病机制和基因治疗提供理论依据。Objective To explore the expression of TGF-β_1 mRNA and protein in patients with hepatocellular carcinoma.Methods The expression of TGF-β_1?TβRⅡ protein in 36 cases of HCC and surrounding HCC tissue was separately detected using immunohistochemistry technique.The expression of TGF-β_1mRNA in 26 cases of HCC and surrounding HCC tissue was separately detected using the method of in situ hybridization.The relationship between TGF-β_1 mRNA and TGF-β_1 protein,TGF-β_1 mRNA and TβRⅡ protein was observed.Results The expression of TGF-β_1mRNA and TGF-β_1 protein in HCC tissue was higher than that in surrounding HCC tissue( P< 0.01).But TβRⅡ protein was in opposite way( P< 0.01).There was significant positive relativity between TGF-β_1 protein and TGF-β_1 mRNA,but the expression of TβRⅡ protein was negatively correlated with that of TGF-β_1 mRNA in HCC tissue.Conclusions Variation on TGF-β_1 gene may be associated with occurrence of HCC.The decline of inhibition of TGF-β_1 was due to the decline of expression of TβRⅡ protein.These results can provide the theoretical basis of the pathogenesis and gene therapy of HCC.

关 键 词：肝细胞癌 癌组织 TGF-Β1基因 mRNA 蛋白表达 原位杂交法 免疫组化技术 基因治疗 发病机制 

分 类 号：R735.7[医药卫生—肿瘤]