用鼻咽相对特异性调控区建立N-LMP1转基因小鼠  

Construction of N-LMP1 Transgenic Mice with the Specific Regulation Region in Nasopharynx

作　　者：张玲[1] 蓝轲[1] 冯湘玲[1] 乔贵林[2] 沈新民[2] 石益民[2] 李虹[1] 姚开泰[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙410078 [2]第一军医大学病理教研室,广州510515

出　　处：《生物化学与生物物理学报》2003年第12期1072-1076,共5页

基　　金：国家自然科学基金重点项目 (No .3 973 0 2 0 0 );国家自然科学基金项目 (No .3 9970 3 0 6);国家重点基础研究发展规划项目 ( 973计划 )(No.G19980 5 12 0 1)资助~~

摘　　要：为了研究EBV LMP1在鼻咽癌发生发展中的作用 ,构建了EDL 2、PLUNC p双启动子调控鼻咽癌来源的LMP1(latentmembraneprotein 1,潜伏膜蛋白 1)的表达载体 ,采用受精卵前核显微注射法构建转基因小鼠。结果表明 ,在所获得的 5 8只转基因首建鼠中 ,4只整合阳性 ,其中的一只转基因小鼠在鼻咽、前胃、舌根等部位检测到了外源基因的表达。In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.

关 键 词：鼻咽癌 EBV-LMP1 组织相对特异性渊控区 N-LMP1 转基因小鼠 潜伏膜蛋白l 表达载体 

分 类 号：R-332[医药卫生]