PCR-ELISA法检测尿脱落细胞端粒酶活性在临床上的意义  

Detection of telomerase activity by PCR-ELISA in exfoliated cells in bladder carcinoma and its value in the diagnosis of bladder carcinoma

作　　者：马小兵[1] 何晓文[2] 章益峰[1] 瞿佩飞[1] 纪家涛[1] 

机构地区：[1]解放军第四一一医院,上海200081 [2]海军医学研究所,上海200433

出　　处：《海军医学杂志》2004年第1期4-5,共2页Journal of Navy Medicine

摘　　要：目的 :探讨尿中脱落细胞端粒酶阳性表达在膀胱上皮肿瘤诊断、术后复发判断中的作用。方法 :应用PCR ELISA法检测 33例膀胱移行上皮肿瘤患者以及 5例正常对照者脱落细胞端粒酶活性。结果 :33例膀胱上皮细胞肿瘤患者中30例尿液脱落细胞端粒酶活性阳性 ,阳性率为 90 .91% ,5例健康对照组检测均为阴性 ,统计学差别极显著 (P <0 .0 1)。端粒酶活性与肿瘤的分期和分级无关联。结论 :尿脱落细胞端粒酶活性测定 ,对早期诊断膀胱上皮肿瘤和疗效预后判断是高敏感而有效的方法。Objective: To assess telomerase activity of exfoliated cells in urine so as to find a new noninvasive method for early diagnosis of bladder carcinoma and detection of recurrence after surgery. Methods: Telomerase activity of exfoliated cells in urine was detected by using PCR-ELISA in 33 patients with bladder carcinoma and 5 healthy volunteers were used as controls. Results: Telomerase activity of exfoliated cells in urine was detected in 30 of the 33 (90.91%) bladder carcinoma patients. In 5 healthy volunteers, no telomerase activity was detected(P<0.01) .No association could be found between telomerase activity and tumor stage or tumor grade. Conclusion: The detection of telomerase activity of exfoliated cells in urine is a highly sensitive and effective method for early diagnosis and detection of recurrence after surgery in patients with bladder carcinoma.

关 键 词：膀胱肿瘤 端粒酶 PCR-ELISA法 

分 类 号：R730.43[医药卫生—肿瘤]