烟草青枯病菌(Pseudomonas solanacearum)细菌素的生物学特性研究  

Some Characteristics of Bacteriocin Produced by Pseudomonas solanacearum strain from Tobacco

作　　者：张建华[1] 郑继发[1] 许永玉[1] 王智发[1] 

机构地区：[1]山东农业大学植保系

出　　处：《中国烟草》1992年第2期6-9,共4页China Tobacco

摘　　要：本文研究了烟草青枯病菌(Pseudomonas solanacearum)无毒产细菌素菌株 A_(3-5)产生的细菌素的生物学特性,结果表明,用不同制备方法提取的 A_(3-5)的细菌素,Au 值均为8。失活温度为60℃,在30℃以下温度放置25天抑菌活性不变,35℃下放置20天,活性开始减弱,40℃放置10天,活性即减弱,25天对几乎完全失活。这说明 A_(3-5)的细菌素虽具有较强耐热能力,但高温不利于其存活。此外,0.5%的胰蛋白酶即刻使其完全失活,说明对胰蛋白酶敏感。经1万转/分钟和5万转/分钟连续离心后,细菌素仍存在于上清液中,说明为非沉淀性的。以上特性表明 A_(3-5)的细菌素可能是一种小分子物质。The crude suspension of bacteriocin produced by a avirulent bacteriocin producer strain (ABPS),A3- 5,of Pseudamonas solanacearum was prepared for the study of biological characteristics.Results showed that the bacteriocin produced by A3-5 with different culture methods was 8 Au/ml.The bacteriocin was inacti- vated at 60℃ for ten minutes,howeves,stable at or below 30℃ for twenty five days,35℃ for twenty clays and 40℃ for ten days.The bacteriocin was almost inactivated completely at 40℃ for twenty five days or after the tratment with 0.5% trypsin at 25℃ for one hour.After the centrffugation of crude preparation of the bacteriocin at 10 and 5×10rpm respectively,the inhibition of the bacteriocin to bacteria was detected only in the supernants.It is suggested that the bacteriocin produced by ABPS A3-5 is a kind of low molecu- lar substanee.

关 键 词：细菌素 烟草 青枯病 生物学特性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]