人类乳腺良恶性病变的SSTR2mRNA表达及其与类固醇受体相关性研究  

A study on SSTR2 mRNA expression and its correlation with steroid receptors in human benign and malignant breast tissues

作　　者：曾希志[1] 姚榛祥[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《肿瘤》2004年第2期135-138,共4页Tumor

摘　　要：目的　了解人类良、恶性乳腺病变组织中生长抑素受体亚型 2 (SSTR2 )mRNA的表达情况 ,并探讨其与雌激素受体(ER)、孕激素受体 (PR)的相关性。方法　收集 2 3例乳腺癌、16例乳腺增生病和 9例乳腺纤维腺瘤手术标本 ,采用多相寡核苷酸探针原位杂交法检测SSTR2mRNA表达 ,免疫组化法检测ER、PR状况 ,并用图像分析仪进行SSTR2mRNA相对含量测定。结果　SSTR2mRNA在乳腺癌表达的阳性率 (87% )和相对含量 (0 .4 7)均明显高于乳腺良性病变 (6 4 % ,0 .2 6 ) (P <0 .0 5 )。乳腺良性病变中SSTR2mRNA表达与ER呈正相关 (P <0 .0 5 ) ;在乳腺癌中SSTR2mRNA表达与ER、PR均呈正相关(P <0 .0 5 )。结论　SSTR2mRNA在乳腺良、恶性病变组织中普遍表达 ,恶性高于良性 ;SSTR2mRNA表达与ER、PR具有相关性 ,提示对ER阳性乳腺癌采用抗雌激素与SST类似物联合治疗的可行性。Objective To investigate somatostatin receptor subtype 2 (SSTR2) mRNA expression and its correlation with steroid receptors in benign and malignant breast tissues. Methods A total of 23 breast carcinomas,16 mammary hyperplasia and 9 mammary adenofibroma samples were analysed. The SSTR2 mRNA expression was examined by in situ hybridization using multiphase oligoprobes. The ER and PR were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative contents of SSTR2 mRNA. Results The positive rate of expression of SSTR2 mRNA (87.0%) and the relative contents(0.47) in breast cancer were higher than those in benign breast tissues(64%,0.26) ( P <0.05). SSTR2 mRNA expression was closely correlated with ER and PR in breast cancer( P <0.05),A positive correlation between SSTR2 mRNA expression and ER was also found in benign breast tissues. Conclusion SSTR2 mRNA expression was ubiquitous in both benign and malignant breast tissues,but higher in malignant than in benign tissues. There is a significant positive correlation of SSTR2 mRNA expression with ER or PR. These results suggest that conbination treatment with an antiestrogen and a somatostatin analogue to ER-positive breast cancer is feasible.

关 键 词：乳腺肿瘤 生长抑素受体亚型2 雌激素受体 孕激素受体 临床意义 原位杂交法 免疫组化法 

分 类 号：R737.9[医药卫生—肿瘤]