WT1基因对肾胚瘤细胞系G401细胞肿瘤逆转的研究  

WT1 expression alters tumorigenicity of the G401 cell line.

作　　者：胡兰[1] 姜又红[1] 舒红[1] 魏雷震[1] 任常山[1] 

机构地区：[1]中国医科大学肿瘤研究所,辽宁沈阳110001

出　　处：《现代肿瘤医学》2004年第2期92-94,共3页Journal of Modern Oncology

摘　　要：目的　肾胚瘤是一种较常见的儿科实体肿瘤 ,该肿瘤的发生与抑癌基因WT1密切相关。本研究的目的是利用脂质体介导转染法将WT1cDNA导入G4 0 1细胞 ,研究WT1基因对G4 0 1细胞生长凋亡及致瘤性的作用。方法　通过Westernblot检测WT1基因在肾胚瘤中的表达、采用碘化丙锭 (PI)染色法检测G4 0 1细胞的凋亡情况、并且对G4 0 1的致瘤性进行分析 ,探讨WT1基因在肾胚瘤发生及治疗中的作用。结果　WT1cDNA可诱导G4 0 1细胞凋亡 ,并且使其致瘤性有所降低。结论　该结果为确定WT1在肾胚瘤病因方面的作用。Objective Wilms tumor is one of the most common solid tumors in children.Some studies have implicated a loss of WT1 tumor suppressor gene function in the development of Wilms'tumor.The objective was to determine the potential biological consequences of WT1 in these tumors.Methods We transfected WT1 cDNA into the pediatric kidney-derived cell line G401.Ddetecting WT1 gene expression by Western blot,tumorigenicity and apoptosis of G401 cell line. Results Introduction of this gene caused a significant suppression of tumorigenicity in the cells and apoptosis cell number increased.Conclusion These data indicafe the concept for a central role for WT1 inactivation in the etiology of this disease.

关 键 词：WT1基因 肾胚瘤 G401细胞系 抑癌基因 脂质体 细胞凋亡 小儿 

分 类 号：R737.11[医药卫生—肿瘤]