散发性大肠癌微卫星不稳定性的研究  

Study of Microsatellite Instability in Sporadic Colorectal Cancers

作　　者：胡家萍[1] 余永欢[1] 戴安邦[1] 

机构地区：[1]江西医学院第一附属医院普外科,江西南昌330006

出　　处：《江西医学院学报》2004年第2期30-33,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的　探讨散发性大肠癌 (SCRC)微卫星不稳定性 (MSI)与临床病理特征和预后的关系。方法　选择 5个微卫星位点 ,采用PCR -银染法检测 5 0例散发性大肠癌石蜡组织的微卫星不稳定性。结果　根据出现MSI的位点数多少 ,SCRC分为 3型 :MSI H肿瘤 (n =8,16 % ) ;MSI L肿瘤 (n =7,14 % )和MSS肿瘤 (n =35 ,70 % )。SCRC3种表型在临床病理特征方面相互比较时 ,MSI H肿瘤与肿瘤部位、年龄、组织类型及肿瘤淋巴细胞浸润有相关 (P <0 .0 5 ) ,而MSI L和MSS比较其差别均无显著性 (P >0 .0 5 )。SCRC3种表型与性别、Dukes分期、淋巴结转移、浸润深度、组织学分级和生存率均无相关 (P >0 .0 5 )。结论　在临床病理特征方面 ,MSI H肿瘤明显不同于MSI L和MSS肿瘤 ,而MSI-L和MSS肿瘤没有明显差别。Objective To study the relation between microsatellite instability(MSI)and clinicopathological features and prognosis of sporadic colorectal carcinoma(SCRC).Methods Fifty archival paraffin embedded specimens resected from patients with primary SCRC were examined for MSI using PCR-sliver nitrate staining analysis and five microsatellite markers.Results Based on the number of markers displaying instability per tumor,SCRC was classified into three groups:high-frequency MSI tumor(MSI-H,n=8,16%);low-frequency MSI tumor (MSI-L,n=7,14%)and microsatellite stability tumor(MSS,n=35,70%).Comparing across the three groups in clinicopathological features,the MSI-H tumors were associated with tumor site,age, histologic type and tumor lymphocytic infiltration(P<0.05),whereas no significant difference was noted between the MSI-L and MSS group(P>0.05).Furthermore, there was not any association of SCRC phenotype with sex,Dukes stage,lymph node metastasis, depth of invasion,grade and survival rate (P>0.05) in either of three groups.Conclusion The results suggested that the MSI-H phenotype cancer is distinct from both MSI-L and MSS phenotype cancers in clinicopathological features, while there is not apparent difference of the features between MSI-L and MSS. MSI may not be a indicator of prognosis in SCRC.

关 键 词：散发性大肠癌 微卫星不稳定性 临床病理 预后 

分 类 号：R735.34[医药卫生—肿瘤]