大鼠脑缺血再灌注时小胶质细胞活化与外源性巨噬细胞补充(英文)  

Activation of microglia and recruitment of extraneous macrophage following ischemia-reperfusion in adult rats

作　　者：袁琼兰[1] 邓莉[1] 高小青[1] 李瑞祥[2] 羊惠君[2] 张光鹏[2] 

机构地区：[1]泸州医学院神经生物学研究室,四川省泸州市646000 [2]四川大学华西医学中心解剖学教研室,四川省成都市610041

出　　处：《中国临床康复》2004年第10期1949-1951,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察脑缺血再灌注时小胶质细胞的活化和外源性巨噬细胞来源。方法:阻塞大鼠大脑中动脉2h再灌注0.5～48h制成脑缺血模型,凝集素GSI-B4组化法观察小胶质细胞/巨噬细胞在脑组织的分布、免疫组化观察室管膜细胞增殖及分化。结果:再灌注0.5h,可见大量形态各异的GSI-B4阳性的小胶质细胞与巨噬细胞,随后下降,12～48h数量持续增高,并呈不同的形态。各实验组,室管膜及室管膜下层有细胞增殖核抗原(proliferationcellnuclearantigen,PCNA)强阳性细胞。杏仁核、杏仁皮质核、视前区及其软脑膜有PCNA强阳性细胞和GSI-B4强阳性阿米巴样巨噬细胞。再灌注24h组,有PCNA与白细胞共同抗原45RB(leukocytecommonantigen,CD45RB)双标阳性的细胞。结论:缺血再灌注时,不同损伤区域小胶质细胞形态呈明显的异形性,外源性巨噬细胞可能来自脑膜巨噬细胞和室管膜及室管膜下层的增生、浸润和迁移。AIM:To study the activation and proliferation of microglia/ macrophages in cerebral tissue in rats following ischemia reperfusion. METHODS: The ischemia model was established by blocking middle cerebral artery for 2 hours and reperfusing for 0.5-48 hours,and control sham operated(n=3) and normal(n=3) rats were processed.The immunocytochemical and lectin GSI B4 by single and double staining were used to investigate temporal profile and morphological characteristics of microglia/macrophages after induction of focal cerebral ischemia in adult rats. RESULTS: There were many GSI B4 positive microglias/macrophages with different morphologies as early as within 0.5 hour.The number of positive cell declined from 3 to 6 hours and increased again from 12 to 48 hours.The GSI B4 positive macrophages were located in the superficial layer of preoptic area and cortical amygdaloid nucleus and meninges covering them.In control groups and all ischemia reperfusion groups,PCNA positive immunohistochemistry was present in the ventricular and subventricular zones(VZ/SVZ).Moreover,the strong PCNA immunoreactivity and GSI B4 strong positive amoebic like macrophages were present in amygdaloid nucleus, cortical amygdaloid nucleus and meninges covering them.The double staining for PCNA and CD45RB existed the same cell from 24 to 48 hours. CONCLUSION:In ischemia reperfusion,the microglia showed distinctly different morphologies.The extraneous macrophages may deride from not only the proliferation,immigration of macrophages in meninges,but also from the proliferation,immigration and differentiation of VZ/SVZ.

关 键 词：大鼠 脑缺血 再灌注损伤 小胶质细胞活化 外源性巨噬细胞补充 动物模型 

分 类 号：R743.31[医药卫生—神经病学与精神病学]