肝气郁结证、肝火上炎证与5-HTT、TPH基因多态性相关研究  

Association Study for Serotonin Transporter and Tryptophan Hydroxylase Gene Polymorphisms and Hepatic Depression Syndrome and Hyperpyrexia of Liver Syndrome Disorder

作　　者：胡随瑜[1] 尤劲松[1] 张宏耕[2] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008 [2]湖南省脑科医院神经症科,湖南长沙410007

出　　处：《中国中医基础医学杂志》2004年第3期49-52,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目 (3 0 0 70 95 3 )

摘　　要：目的 :探讨肝气郁结证、肝火上炎证与五羟色胺转运体 (5 HTT)、色氨酸羟化酶 (TPH)基因三种多态性的相关性。方法 :运用多聚酶链式反应技术 (PCR)检测 5 0例肝气郁结证、5 0例肝火上炎证患者与 90例健康对照者三种基因多态性的分布频率。结果 :肝火上炎证患者 5 HTT启动子区多态性 (5 HTTLPR)的SS(short short)基因型和S(short)等位基因频率分别为 72 %和 85 %,健康对照者SS基因型和S等位基因频率分别为 4 9%和 71%,两组之间比较差异有显著性 (P <0 0 5 )。肝气郁结证患者 5 HTTLPR的SS基因型和S等位基因频率分别为 5 8%和 74 %,与健康组比较差异无显著性。 5 HTT内含子 2可变数串联重复序列 (VNTR)多态性和TPH基因A2 18C多态性各基因型和等位基因频率在肝气郁结证、肝火上炎证患者组与健康人组中均差异无显著性 (P >0 0 5 )。结论 :5 HTTLPR多态性SS型个体可能是肝火上炎证的易感人群。Objective:To explore the relationship between the hepatic depression syndrome and hyperpyrexia of liver syndrome disorder and polymorphisms in the serotonin transporter (5 HTT) and tryptophan hydroxylase(TPH).Methods:Using a polymerase chain reaction based technique,the frequencies of 5 HTTLPR,5 HTT intron2 VNTR,and TPH intron7 A218C polymorphisms were determined in patient groups and control subjects.Genotype and allele frequency comparisons between patient groups and control subjects were carried out with chi square tests or Fisher's exact tests.Results:The frequencies of 5 HTTLPR short/shor(SS) genotype were significantly higher in patients with hyperpyrexia of liver syndrome than in control subjects(72% vs 49%, P =0 026),and the S allele frequencies were significantly higher in patients with hyperpyrexia of liver syndrome than in control subjects(85% vs 71%, P =0 009).However,there were no differences for the distribution of 5 HTTLPR between patients with deficiency of hepatic Qi syndrome and controls.No significant differences for the genotype distribution or the allele frequency of 5 HTT intron2 VNTR and TPH intron7 A218C,polymorphisms were revealed when comparing controls and all patient groups.Conclusion:This finding support the presence of 5 HTTLPR SS genotype may increase the risk of hyperpyrexia of liver syndrome.

关 键 词：肝气郁结证 肝火上炎证 5-HTT TPH 基因多态性 

分 类 号：R228[医药卫生—中医基础理论]