脉络膜黑色素瘤微血管密度与其临床组织病理学预后指标关系的探讨  

Correlation of tumor microvessle density with clinicopathologic prognostic parameters in choroidal melanoma

作　　者：王凤华[1] 李彬[1] 李辽青[1] 孙宪丽[1] 陈长喜[1] 

机构地区：[1]首都医科大学北京同仁眼科中心北京市眼科研究所,北京100730

出　　处：《眼科研究》2004年第1期17-20,共4页Chinese Ophthalmic Research

摘　　要：目的探讨脉络膜黑色素瘤中微血管密度(MVD)与临床病理学预后指标及巩膜导管受侵与否的关系。方法应用高碘酸Schiff(PAS)特殊染色法显示血管基底膜,免疫组化染色法检测65例脉络膜黑色素瘤中血管内皮细胞特异性标记物第八因子相关抗原(FⅧ-RAg)的表达,并通过形态学计量法计数肿瘤内微血管密度。结果肿瘤微血管密度与脉络膜黑色素瘤患者年龄、肿瘤位置、肿瘤高度无关,与肿瘤最大基底直径(LTD)、肿瘤大小分组、细胞类型、侵犯巩膜导管与否有关。结论肿瘤血管增生与临床病理学预后指标之间有密切关系,提示MVD可能成为预测脉络膜黑色素瘤预后的重要指标之一。Objective To investigate t he relationship between microvessel density(MVD)and clinicopathologic prognost ic predictors in choroidal melanoma.Methods Sixty-five cases of choroidal mel anoma were selected.MVD was identified by periodic acid Schiff(PAS)staining of periendothelial basement membrane without hematoxylin counterstaining and b y immunohistochemical staining of vascular endothelial cells with factorⅧ-rela ted antigen(FⅧ-RAg).Tissure sections stained by PAS were examined with a gr een narrow band pass filter at540nm and produced a magenta color in tumor vas cular.Results There was no correlation between the MVD and tumor location,as w ell as the tumor height and the patient ’s age(P>0.05).However,signif icant correlation was found between the MVD and the largest tumor diameter(LTD ),as well as tumor size,cell type and tumor invasion to the scleral emissaria (P<0.01).Conclusion Angiogenesis is tightly correlated with clinicopatho logic prognostic factors,such as LTD,tumor size,cell type and invasion.MVD m ay be a useful prognostic indicator in choroidal melanoma.

关 键 词：脉络膜黑色素瘤 微血管密度 组织病理学 预后 

分 类 号：R739.7[医药卫生—肿瘤]