IL-1、TNF-α和IFN-γ对Grave’s眼病患者与正常人眼眶成纤维细胞的作用  

Effects of IL-1,TNF-α and IFN-γ on human orbital fibroblast in patient with Graves’ophthalmopathy and normal subject

作　　者：张学进[1] 李涛[1] 罗清礼[1] 

机构地区：[1]四川大学华西医院眼科,成都610041

出　　处：《眼科研究》2004年第1期70-72,共3页Chinese Ophthalmic Research

基　　金：国家教育部博士点基金(C200057);四川省科技厅基金(01SG046-11)资助。

摘　　要：目的观察细胞因子对Graves眼病(GO)患者与正常人眼眶成纤维细胞(OFs)的作用,探讨GO的发病机制。方法取5例GO患者与4例正常对照者眼眶结缔组织与眼外肌进行OFs的培养,分别在其中加入不同浓度的白介素1(IL-1)、肿瘤坏死因子α(TNF-α)、干扰素α(IFN-α),干扰素γ(IFN-γ),用四甲基偶氮唑盐(MTT)法与放射免疫法检测GO患者与正常人OFs的增殖及分泌透明质酸(HA)的情况。结果IL-1与IFN-γ为5、50、500U/mL时,均能刺激OFs的增殖和HA的分泌,为500U/mL时,对GO患者OFs的刺激作用强于对正常人OFs的作用;TNF-α在50U/mL和500U/mL时能刺激OFs的增殖和HA的分泌,IFN-α在3种浓度均不能刺激OFs的增殖和HA的分泌。结论细胞因子(IL-1、TNF-α、IFN-γ)能刺激OFs的增殖和HA的分泌。细胞因子的紊乱可能参与了GO的发病。Objective To observe the effects of interleuk in (IL)-1,tumor necrosis factor alpha (TNF-α)and interferon gamma(IFN-γ )on proliferation of orbital fibroblasts(OFs)and production of hyaluronic aci d(HA)in vitro.Methods OFs from5patients with Graves’ophthalmopathy(GO)a nd4normal control subjects were cultured in vitro.Different concentrations of IL-1,TNF-α,IFN-αand IFN-γwere incubated with cultured OFs.Proliferat ion of OFs and production of HA were measured by MTT and radioimmunological ass ay.Results Proliferation of OFs and production of HA were significantly increa sed with stimulation by IL-1(5,50,500U/ml),TNF-α(50,500U /ml)and IFN-γ(5,50,500U/ml)respectively,but not by IFN-α.At the concentration of500U/ml,IL-1or IFN-γcould stimulate OFs of patien ts to produce more HA in comparison with control subjects.Conclusion IL-1,TN F-αand IFN-γare stimulators to proliferation of OFs and HA production.The disturbance of cytokines may be involved in the GO pathogenesis.

关 键 词：IL-1 TNF-α IFN-Γ Grave’s眼病 眼眶 成纤维细胞 

分 类 号：R771.3[医药卫生—眼科] R581.1[医药卫生—临床医学]