BRI基因在人非小细胞肺癌细胞系中的差异表达  

Differential expression of BRI gene in human non-small cell lung cancer cells

作　　者：陈英准[1] 李钰[2] 邹嵘[2] 陈宇[2] 冯会臣[2] 王吾如[3] 

机构地区：[1]哈尔滨医科大学附属第二医院病理科,150086 [2]哈尔滨医科大学基础医学院生物教研室 [3]哈尔滨医科大学基础医学院病理教研室

出　　处：《中国肺癌杂志》2004年第1期12-15,共4页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金 (No .39970 396和No.30 1 70 51 6)资助~~

摘　　要：目的　研究BRI基因在人非小细胞肺癌细胞系中的表达 ,探讨其表达情况与肺癌转移能力的相关性。方法　应用半定量RT PCR、Northernblot法分析一对来源相同、转移能力不同的人肺腺癌细胞系AGZY83 a和Anip973中BRI基因的差异表达 ,并检测另外 6个肺癌细胞系SPC A 1、A5 49、95D、TKB 18、GLC 82和PAa中BRI基因的表达情况 ,分析其过度表达与肺癌转移能力的关系。结果　BRI基因在两同源细胞系中确实存在明显的差异表达 ,在具有高转移潜能的Anip973中呈高表达。在其他 6个不同转移潜能的肺癌细胞系中BRI的表达情况也与其转移能力有一定的相关性。BRI基因存在 1.6kb和 2 .0kb两个转录本 ,在肺癌细胞系中以 1.6kb转录本为主。结论　BRImRNA尤其是 1.Objective To investigate the correlation between BRI gene expression and the metastatic potential in human non small cell lung cancer cell lines. Methods BRI gene differential expression was detected between human lung adenocarcinoma cell lines AGZY83 a and Anip973 by RT PCR and Northern blot. Anip973 was isolated from AGZY83 a with a higher metastatic potential than its parent line. Other 6 human non small cell lung cancer cell lines, A549, TKB 18, SPC A 1, GLC 82, 95D and PAa, were also detected for the relationship between BRI expression and metastatic potential. Results There was a significant difference in BRI gene expression between AGZY83 a and Anip973 cell lines. BRI was overexpressed in Anip973 cells comparing to AGZY83 a cells. Up regulation of BRI gene was also observed in other 6 lung cancer cell lines, and partly correlated with their metastatic potential. Furthermore, there were two mRNA transcripts in the lung cancer cells, in which the 1.6 kb transcript was the major one. Conclusion The up regulated expression of BRI 1.6 kb mRNA transcript may indicate the formation of metastatic potential of NSCLC. BRI is possibly a metastasis related gene.

关 键 词：BRI基因 非小细胞肺癌 癌细胞 基因表达 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤] R394[医药卫生—临床医学]