维拉帕米对牛眼小梁细胞表达转化生长因子-β的影响  

The effect of verapamil on expression of transforming growth factor-β in bovine trabecular meshwork cells

作　　者：陈飞[1] 魏厚仁[1] 曾艳彩[1] 吕源淑[1] 张樱[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022

出　　处：《眼科研究》2004年第2期156-160,共5页Chinese Ophthalmic Research

摘　　要：目的　研究维拉帕米 (Verapamil)在抑制牛眼小梁细胞 (BTMC)合成细胞外基质 (ECM )的同时 ,对BTMC表达转化生长因子 β1(TGF β1)、TGFβ2 的影响。方法　采用半定量RT PCR和免疫组化结合计算机图像分析技术分别检测等于和低于 0 0 1mg/mLVerapamil对BTMC表达TGFβ1、TGFβ2 蛋白和mRNA。结果　 0 0 0 1、0 0 0 5、0 0 1mg/mLVerapamil可质量浓度依赖性抑制BTMC表达TGFβ1、TGFβ2 蛋白和mRNA(P <0 0 5 ) ,且 0 0 1mg/mLVerapamil可完全抑制TGF β1的蛋白合成 ,同样质量浓度的Verapamil对TGF β1的抑制程度要大于TGF β2 (P <0 0 5 )。结论　Verapamil抑制BTMC合成ECM的机制可能为抑制TGF β表达 ,这种抑制是在转录水平上 ,且对TGF β1的抑制可能在其中起主要作用。此外 ,TGF β可通过自分泌方式作用BTMC ,促进ECM的合成 ,进一步为Verapamil在发病学环节上防治原发性开角型青光眼提供实验依据。ObjectiveTo study the effect of verapamil on the expression of transforming growth factor-β 1(TGFβ 1),TGF-β 2 and extracellular marix(ECM) synthesis in bovine trabecular meshwork cells(BTMC). MethodsThe effect of verapamil equal to or less than 0.01 mg/mL on the expression of TGF-β 1,TGF-β 2 in BTMC was detected by immunohistochemistry with computer-aided color image analysis and semi-quantitative RT-PCR analyses, respectively.Results0.001,0.005 and 0.01 mg/mL of verapamil could inhibit the expression of TGF-β 1,TGF-β 2 in BTMC at the level of protein and mRNA in a concentration-dependent manner(P<0.05),and 0.01 mg/mL verapamil could inhibit the protein synthesis of TGF-β 1completely.The same concentration of verapamil inhibited the expressions of TGF-β 1 more than TGF-β 2.ConclusionThe possible mechanism for inhibiting the ECM synthesis by verapamil may be related to decreasing TGF-β expression,and the inhibition of TGF-β 1expression may play a principle role.This result further provides an experimental basis for the prospect of pathogenetic treatment of primary open-angle glaucoma(POAG) by verapamil.

关 键 词：维拉帕米 小梁细胞 表达 转化生长因子-Β 原发性开角型青光眼 

分 类 号：R775[医药卫生—眼科]