热休克蛋白60、70在口腔扁平苔藓中表达的研究  

Studies of heat shock protein 60 and heat shock protein 70 in oral lichen planus

作　　者：邸萍[1] 高岩[1] 

机构地区：[1]北京大学口腔医学院口腔病理研究室

出　　处：《中华口腔医学杂志》2003年第4期275-278,I004,共5页Chinese Journal of Stomatology

摘　　要：目的　探讨热休克蛋白 (heatshockprotein ,HSP) 60和HSP70在口腔扁平苔藓病变中的作用。方法　对 62例口腔扁平苔藓、1 0例正常口腔粘膜、2 1例慢性盘状红斑狼疮、1 0例粘膜良性淋巴组织增生病 ,46例白斑进行免疫组织化学SP法染色 ,分析HSP60、HSP70在口腔扁平苔藓中的表达 ;对 1 2例口腔扁平苔藓和 5例正常粘膜进行逆转录PCR实验 ,观察HSP60、HSP70mRNA的变化。结果　HSP60在口腔扁平苔藓病损区的表达较其他各组显著增强 ,差异有显著性 (P <0 0 0 1 )。HSP70在糜烂型口腔扁平苔藓病损区的表达下调。RT PCR结果显示 ,HSP60、HSP70mRNA表达增强。Objective To study the role of heat shock protein(HSP)60 and HSP70 in the pathogenesis of oral lichen planus(OLP) Methods The immunohistochemical SP methods were used to detected the expression of HSP60 and HSP70 in 62 cases of oral lichen planus, 10 cases of normal oral mucosa, 21 cases of chronic discoid lupus erythematosus (DLE), 10 cases of benign lymphoadenosis of mucosa, 46 cases of leukoplakia RT PCR was used to evaluate the expression of HSP60 mRNA and HSP70 mRNA in 12 cases of OLP and 5 cases of normal oral mucosa Results HSP60 was expressed at much higher levels in OLP than other groups The expression of HSP70 decreased in epithelial cells in erosive lichen planus group HSP60 mRNA and HSP70 mRNA were expressed at high level in OLP Conclusion HSP60 and HSP70 play an important role in pathogenesis of OLP

关 键 词：热休克蛋白 口腔扁平苔藓 HSP 基因表达 口腔粘膜 LP 免疫组化 

分 类 号：R781.5[医药卫生—口腔医学]