肿瘤坏死因子和脂多糖影响牙周膜细胞表达骨保护因子的研究  

Tumor necrosis factor and lipopolysaccharide affect periodontal ligament cells expressing osteoprotegerin in vitro

作　　者：李京平[1] 凌均棨[2] 

机构地区：[1]中山大学光华口腔医学院牙周科,广州510060 [2]中山大学光华口腔医学院牙体牙髓科,广州510060

出　　处：《中华口腔医学杂志》2003年第4期288-291,I005,共5页Chinese Journal of Stomatology

摘　　要：目的　研究脂多糖 (lipopolysaccharide,LPS)和肿瘤坏死因子α(tumornecrosisfactor α,TNF α)对牙周膜细胞增殖及分泌骨保护因子 (osteoprotegerin ,OPG)的影响。 方法　培养牙周膜细胞并形成单细胞克隆 ,培养基中加入LPS和TNF α ,MTT法检测牙周膜细胞增殖水平 ,SandwitchELISA法测定培养上清液中OPG含量。结果　TNF α浓度在 1 0 μg/L以上时可增加细胞对OPG的表达量 (P <0 0 5) ,但由于TNF α同时抑制牙周膜细胞增殖 (P <0 0 5 ) ,因此培养上清中OPG总量无明显变化 (P >0 0 5) ;LPS对牙周膜细胞增殖和OPG表达均无明显影响 ,与TNF α也没有交互作用 (P >0 0 5)。结论　TNF α能刺激牙周膜细胞OPG表达水平的提高 ;Objective To investigate the effects of LPS and/or TNF α on periodontal ligament cell (PDLC) proliferation and OPG secretion Methods Healthy premolars extracted for orthodontic reasons from a 12 years old boy were obtained, and periodontal tissues were collected and cultured to obtain PDLCs Cloned PDLCs were obtained by means of limited dilutions, and were characterized as follows: alkaline phosphatase activity, collagen Ⅲ production and bone like nodules formation LPS and rhTNF α were added into culture media and their effects on PDLC proliferation and OPG secretion were observed The OPG concentrations in cell culture supernatants were detected by sandwich ELISA Living cell numbers were demonstrated by MTT test The average levels of OPG secretion by a single cell were calculated by dividing OPG concentration with MTT result Results rhTNF α above 10 μg/L decreased the mtt and opg detecting results, but increased the opg/mtt values ( P <0 05) However, LPS had no effect on mtt, opg or opg/mtt values Neithor it had any interaction with rhTNF α( P >0 05) Conclusions TNF α prohibits the proliferation of PDLCs but enhances their OPG secretion However, LPS has no effect on neithor side Our works surport the hypothesis that there may be an inverse feedback regulation pattern of increasing periodontal OPG production against local bone resorption activity PDLCs might not be the natural target cells of LPS′ direct cytotoxic effect

关 键 词：肿瘤坏死因子Α 脂多糖 LPS 牙周膜 细胞表达 骨保护因子 TNP-α 细胞培养 

分 类 号：R781.4[医药卫生—口腔医学]