核心结合因子α1诱导人牙乳头细胞矿化的相关蛋白表达  

Cbfa1 induces the expression of the mineral-related proteins in human dental papilla cells

作　　者：余擎[1] 肖明振[1] 吴补领[1] 朱庆林[1] 郭婷[1] 李峰[1] 

机构地区：[1]第四军医大学口腔医学院牙体病科,西安710032

出　　处：《中华口腔医学杂志》2003年第4期271-274,I004,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金 ( 30 2 714 18);陕西省卫生厅科研基金( 0 2D4 8)资助项目

摘　　要：目的　在人牙乳头细胞中探讨核心结合因子α1 (corebindingfactorα1 ,cbfa1 )能否调控矿化相关蛋白的表达。方法　体外培养人牙乳头细胞 ,转染pcDNA3 cbfa1重组质粒 ,稳定表达cbfa1后检测碱性磷酸酶 (alkalinephosphatase ,ALP)和骨钙素 (osteocalcin ,OC)含量 ,同时采用免疫组化、免疫印迹和PCR等方法观察骨桥素 (osteopontin ,OPN )、骨涎蛋白 (bonesialoprotein ,BSP)、骨连素(osteonectin ,ON)、牙本质基质蛋白 (dentinmatrixprotein 1 ,DMP1 )、牙本质涎蛋白 (dentinsialoprotein ,DSP)和牙本质涎磷蛋白 (dentinsialophosphoprotein ,DSPP)的表达。 结果　建立了稳定表达cbfa1基因和蛋白的人牙乳头细胞模型PC 3 ,发现转染后细胞的ALP和OC含量明显增高 ,OPN、BSP、ON、DMP1的表达也明显增高 ,但未发现牙本质特异性蛋白DSP及其编码基因DSPP的表达。结论　在人牙乳头细胞中 ,cbfa1能上调ALP和OC含量 ,诱导多种矿化组织特异性蛋白的表达 ,在牙齿发育矿化中有重要作用 。Objective To explicit whether the expression of the mineral related proteins is regulated by cbfa1 in human dental papilla cells Methods Human dental papilla cells were cultured in vitro and transfected with pcDNA3 cbfa1 recombinant plasmids After selected with G418 sulfate, a cell clone named PC 3, which could stably express the cbfa1 mRNA and protein, was proved by PCR and western blot Then the amount of ALP and OC and the expression of OPN, BSP, ON, DMP1, DSP and DSPP were detected by immunohistochemistry, Western blot and PCR methods Results We established the human dental papilla cells model PC 3 which could stably express the cbfa1 mRNA and protein Compared with normal cells, a lot of mineral related proteins such as ALP, OC, OPN, BSP, ON, DMP1 were upregulated in PC 3 cells Conclusions In human dental papilla cells, cbfa1 can induce the expression of most mineral related genes and proteins It may implicate that cbfa1 must play a key role during tooth development and mineralization

关 键 词：核心结合因子Α1 牙乳头细胞 细胞矿化 蛋白表达 基因转染 免疫组化 

分 类 号：R780.2[医药卫生—口腔医学]