牙科陶瓷表面粗糙度与细菌粘附的实验研究  

Adhesion of oral microorganisms on dental porcelain polished and glazed

作　　者：王贻宁[1] 温国江[1] 施斌[1] 潘新华[1] 

机构地区：[1]武汉大学口腔医学院修复科,430079

出　　处：《中华口腔医学杂志》2003年第5期342-344,共3页Chinese Journal of Stomatology

摘　　要：目的　比较陶瓷抛光和陶瓷上釉后表面粗糙度的差异及变形链球菌在其表面的粘附能力。方法　制作陶瓷样本 30个。A组陶瓷用金刚石抛光膏打磨抛光 ,B组陶瓷上釉 ,C组陶瓷用2 4 0 #Al2 O3 磨头磨平。用轮廓仪测量各组陶瓷样本的表面粗糙度。将各组样本置于变形链球菌悬浮液中 ,37℃培养 1h。图像分析仪计算变形链球菌在各样本表面的粘附面积比。结果　A、B、C组陶瓷的表面粗糙度分别为 (0 1987± 0 0 5 7) μm、(0 1990± 0 0 91) μm及 (0 4 2 6 0± 0 174 ) μm。变形链球菌在A、B、C 3组陶瓷表面的粘附面积比分别为 (15 92± 4 37) %、(16 39± 6 31) %、(41 4 8± 12 1) %。经统计学分析 ,A、B组陶瓷组间表面粗糙度及细菌粘附面积比差异无显著性 (P >0 0 5 ) ,C组陶瓷与其他各组样本表面粗糙度及细菌粘附面积比差异有高度显著性 (P <0 0 1)。结论　陶瓷上釉和抛光表面能达到同样光滑 ,细菌在其表面的粘附量亦基本相同。Objective This study compared the roughness of porcelain polished or glazed surfaces and the adhesion of oral streptococcus mutans to them in vitro.Methods 30 porcelain samples were made . Porcelain samples in group A were polished with diamond paste. Porcelain samples were glazed in group B and were polished with Al 2O 3 (240#)bur in group C.Their roughness values were measured by profilometer. Standardized cell suspensions were incubated with test samples for one hour at 37 ℃, then retained cells were counted by image analysis(percentage area of a microscopic field covered by cells). Results Roughness values of group A、B、C were respectively (0.198 7±0.057)μm, (0.199 0±0.091)μm, (0.426 0±0.174)μm. There was no significantly difference between group A and group B. The roughness samples in group C were significantly rougher than that in the other groups. The amount of retained cells in groupA、groupB、groupC was respectively (15.92±4.37) %, (16.39±6.31)% and (41.48±12.1)%. There was no significant difference between the cell adhesion on porcelain surface glazed and polished,but more bacteria adhered on the porcelain surface in group C. Conclusions Porcelain surface polished treatment was clinically acceptable compared with its glazed . They all exhibited the least amount of bacteria adhesion. The more porcelain surface was rough,the more bacteria adhered on it.

关 键 词：牙科 陶瓷表面粗糙度 细菌粘附 变形链球菌 口腔修复体 

分 类 号：R783.1[医药卫生—口腔医学] R378[医药卫生—临床医学]