电热电化学联合治疗对S180肉瘤杀伤效果的实验研究  

Antitumor effects of electrothermal and electrochemical therapy on mice with sarcoma180

作　　者：孙才均[1] 谢磊[2] 

机构地区：[1]浙江大学医学院附属第二医院口腔颌面外科,杭州310009 [2]浙江大学医学院附属邵逸夫医院头颈整形联合外科

出　　处：《中华口腔医学杂志》2003年第5期351-354,I005,共5页Chinese Journal of Stomatology

摘　　要：目的　运用新研制的电热电化学治疗仪 ,评价其电热电化学联合治疗对小鼠S180肉瘤杀伤效果 ,并与单纯热疗和单纯电化学治疗比较 ,探讨其可能的作用机制。方法　将负荷S180肉瘤 (直径约 1cm)小鼠随机分成 4组 ,每组 10只。将一对电极沿肿瘤长轴插入。对照组 :不进行任何治疗 ;电化组 :单纯电化学治疗 ;热疗组 :单纯热疗 ;综合组 :电热电化学治疗。治疗后每天计算肿瘤体积。另外取电化组、热疗组和综合组治疗后即刻、6、2 4、72h的肿瘤组织 ,观察其形态学的改变。结果　综合组经电热电化学联合治疗 15min 1次 ,小鼠S180肉瘤能完全被杀灭 ,在 72h后镜下未见肿瘤细胞 ,10d后仍未见肿瘤复发 ,与其他各组比较 ,差异有显著性 (P <0 0 1) ;单纯热疗组和电化组治疗后第 4天分别有 6只和 5只小鼠肿瘤复发。结论　运用新研制的电热电化学治疗仪行电热电化学联合治疗恶性肿瘤的技术是可行的 ,对负荷S180肉瘤小鼠的抑瘤率达 10 0 % ,明显优于单纯电化学治疗和单纯热疗。Objective To explore the anti-tumor effects and the mechanism of a new treatment for Sarcoma180 in mice using both hyperthermia (H) and electrochemical therapy (ECT). Methods A group of mice with Sarcoma180 (1 cm in diameter) were divided randomly into four groups and given different treatments: Control, ECT alone, H alone and electrothermal & electrochemical therapy (ETECT). The volumes of the tumors were calculated everyday after treatment. The tumor tissues were examined morphologically at 0, 6, 24 and 72 hours after treatment. Results The tumors treated with ETECT were completely destroyed, and did not recur within a period of ten days by pathological examination. Results of this group were significantly different with the other groups (P<0.01). However,recurred tumors were found in 6 mice of H group and 5 mice of ECT group, respectively. Conclusions Based on the results obtained, ETECT induced by ETECD(D-device) is an effective way to eliminate cancer. It is capable of completely destroying cancer cells in a short time.The rate of Sarcoma180 inhibition is 100%, and malignant cells can′t recur. The research provided theoretical and experimental bases for the treatment of oral cancer by a new approach to treat oral cancer.

关 键 词：电化学 治疗 S180肉瘤 杀伤效果 电热针 肿瘤 

分 类 号：R73-36[医药卫生—肿瘤]