牙齿形状调控相关基因Wnt6的克隆  

The study on cloning of the related gene Wnt6 regulating teeth patterning

作　　者：郑梁[1] 金岩[2] 肖明振[1] 轩昆[3] 

机构地区：[1]第四军医大学口腔医学院牙体病科 [2]第四军医大学口腔医学院组织病理学教研室 [3]第四军医大学口腔医学院儿童口腔科

出　　处：《临床口腔医学杂志》2004年第4期212-214,共3页Journal of Clinical Stomatology

基　　金：第四军医大学创新基金重点课题资助 (CXO2F0 0 2 )

摘　　要：目的 :获得新生猪牙胚的差异表达基因。方法 :利用消减杂交方法获得新生猪前后恒牙胚的差异表达基因 ,并通过反向点杂交排除假阳性 ,尔后进行测序定性分析。结果 :通过消减杂交获得Wnt6基因。结论Objective:To obtain the differentially expressed genes from subtractive gene library of pig incisor and molar patterning. Method: Differentially expressed gene library was obtained during pig incisor and molar patterning by modified subtractive hybridization technique, then negative genes were eliminated by reverse dot blot .Finally positive genes were sequenced and qualitatively analyzed. Result: the related gene Wnt6 regulating teeth patterning was got .Conclusion : Wnt6 could play the key role in the molecular system regulating teeth patterning.

关 键 词：牙齿形状调控 基因表达 Wnt6 基因调控 恒切牙 

分 类 号：R780.2[医药卫生—口腔医学] R318[医药卫生—临床医学]