鸦胆子油乳对膀胱癌细胞系BIU-87坏死与凋亡的影响  

Necrosis and apoptosis induced by brucea javanica oil emulsion in bladder cancer cell

作　　者：李晓武[1] 王禾[1] 秦卫军[1] 李欣[1] 刘涛[2] 樊春梅[3] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安市710033 [2]第四军医大学基础部解剖教研室,陕西西安市710033 [3]第四军医大学西京医院临床免疫科,陕西西安市710033

出　　处：《中国康复理论与实践》2004年第3期163-164,F003,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：陕西省发展基金 (2 0 0 2K1 0 G4)

摘　　要：目的探讨鸦胆子油乳对膀胱癌BIU 87细胞坏死与凋亡的影响及其可能的作用机制。方法将不同浓度的鸦胆子油乳加入体外培养的BIU 87细胞 ,用流式细胞仪检测细胞凋亡与坏死比例、并检测线粒体膜电位 (MMP) ,用激光共聚焦显微镜观察细胞色素C分布。结果高浓度鸦胆子油乳作用 4h后 ,肿瘤细胞MMP明显降低 ,2 4h后多数细胞发生坏死 ,与对照组相比有非常显著性差异 (P <0 0 1) ;低浓度鸦胆子油乳作用 6h后 ,诱导细胞色素C释放 ,2 4h后可见典型的凋亡现象 ,与对照组相比有显著性差异 (P <0 0 5 )。结论鸦胆子油乳高浓度主要诱导肿瘤细胞坏死 ,低浓度诱导细胞凋亡。Objective To study the effcet of necrosis and apoptosis induced by Brucea javanica oil emulsion on bladder cancer BIU-87 cells. MethodsBrucea javanica oil emulsion of different concentration was added to the bladder cancer BIU-87 cell cultured in vitro. The ratio of apoptosis/necrosis cells and mitochondrial membrane potential(MMP) were detected with flow cytometer. The dissipation of cytochrome C was observed with confocal microscopy. ResultsHigh concentration brucea javanica oil emulsion reduced MMP which led cell necrosis, while that of low concentration diffused the cytochrome C from mitochondria to cytoplasm which induced cell apoptosis. ConclusionHigh concentration brucea javanica oil emulsion mainly causes BIU-87 cells to necrosis, low concentration induces cell to apoptosis.

关 键 词：鸦胆子 膀胱肿瘤 坏死 凋亡 线粒体 细胞色素C 

分 类 号：R737[医药卫生—肿瘤]