血管瘤组织中p63和Fos蛋白的定量表达  

QUANTITATIVE EXPRESSION OF P63 AND C-FOS PROTEIN IN HEMANGIOMA TISSUE

作　　者：刘涓[1] 张端莲[1] 陕声国[2] 陈志雄[1] 余瑛[1] 

机构地区：[1]武汉大学医学院组织胚胎学教研室,武汉430071 [2]武汉大学人民医院整形外科,武汉430060

出　　处：《中国组织化学与细胞化学杂志》2003年第3期276-280,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助 (30 2 71345 ) ;湖北省科技攻关课题资助 (30 1130 6 5 6 )

摘　　要：目的　探讨 p6 3基因和c fos基因的蛋白与血管瘤发生发展的关系。方法　采用免疫组织化学S P法对 4 0例血管瘤和 2 0例正常皮肤组织检测 p6 3基因和c fos基因的蛋白表达 ;对所获得的检测结果进行图像分析处理。结果　在正常皮肤组、增生期与消退期血管瘤中 ,p6 3和Fos蛋白的平均光密度分别为 :0 92 3± 0 191,0 0 79± 0 0 2 4 ;8 2 71±1 95 3,0 12 4± 0 0 15 ;0 92 0± 0 187,0 0 88± 0 0 17。增生期组与消退期组、正常皮肤组分别相比 ,p6 3和Fos阳性表达的差异均有显著性 (P <0 0 5 ) ,消退期组与正常皮肤组之间 ,p6 3阳性表达的差异无显著性 (P >0 0 5 )。结论　p6 3基因在血管瘤中并未作为肿瘤抑制基因起作用 ,相反是作为癌基因而促进内皮细胞的增殖 ,可能与血管形成关系密切。c fos在血管瘤的增生中起着重要作用 ,可能与c fos可通过识别bFGF启动子区的特异位点TRE而启动bFGF基因的转录有关。Objective To investigate the relationship between p63, c-fos proteins and hemangioma development. Method The immunohistochemical method and quantitative image analysis were used to detect p63 and c-fos protein expressions in 40 hemangioma and 20 normal skin tissues. Result In normal skin, proliferative phase and involuting phase, the average light density of p63 and c-fos protein expressions were 0.923±0.191, 0.079±0.024; 8.271±1.953, 0.124±0.015; 0.920±0.187, 0.088±0.017 respectively. The results manifested that both p63 and c-fos expressions in the proliferative phase of hemangioma were significantly different from those in the involuting phase of hemangioma and normal skin, respectively ( P <0.05). But no significant difference in p63 and c-fos expressions was observed between the involuting phase of hemangioma and normal skin ( P >0.05).Conclusion The p63 gene is not a tumor suppressor gene but an oncogene in hemangioma, which may contribute to the proliferation of endothelial cells and be associated with angiogenesis. And c-fos may play an important role in the proliferation of hemangioma and be related with starting the transcription of bFGF gene by recognizing the special site TRE in the bFGF promoter region.

关 键 词：血管瘤组织 P63 FOS蛋白 定量表达 C-FOS基因 P63基因 

分 类 号：R739.5[医药卫生—肿瘤]