海仁酸致痫大鼠海马组织AMPA受体GluR2表达的变化  

EXPRESSION CHANGE OF GluR2 OF AMPA RECEPTORS IN THE HIPPOCAMPUS OF TATS WITH KAINIC ACID-INDUCED SEIZURE

作　　者：魏瑛[1] 刘子建[1] 朱长庚[1] 刘庆莹[1] 

机构地区：[1]华中科技大学同济医学院解剖学系脑研究室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2003年第3期302-305,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助 (30 170 4 94 )

摘　　要：目的　为了研究AMPA受体在癫痫发生中的作用。方法　本研究用免疫组织化学方法观察了海仁酸致痫大鼠海马组织AMPA GluR2受体的表达变化。结果　在侧脑室注射海仁酸后 1h ,4h ,12h ,2 4h及 7d ,大鼠海马CA3区及齿状回GluR2的表达明显减弱 ,显微图像分析 :与对照组相比 ,KA 4h ,KA 12h ,KA 2 4h ,KA 7d组大鼠海马组织GluR2阳性神经元平均光密度值降低 ,差异有显著性 (P <0 0 5 )。结论　在癫痫发作过程中AMPA受体 GluR2亚单位表达改变可能与癫痫发作导致的神经元损伤有密切关系。Objective To explore the epileptogenesis effect of the AMPA receptors. Method Using immunohistochemical methol, the expression change of AMPA receptors GluR2 subunit was observed in the hippocampus of rats with seizure induced by kainic acid (KA). Result After intracerebroventricular injection of kainic acid, the expression of GluR2 was decreased in the hippocampal area CA3 and the dentate gyrus at 1h, 4h, 12h, 24h and 7d. The results of the image analysis and the statistics indicatedthat the average optical density of GluR2-IR cells in theKA 4h, KA 12h, KA 24h, KA 7d groups, was significantly decreased when compared with the control group ( P <0.05). Conclusion The above results suggest that the expression change of AMPA receptors GluR2 subunit may play an important role during seizures.

关 键 词：海仁酸 癫痫 使君子酸受体 大鼠 AMPA受体 GLUR2 表达 神经元损伤 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R322.81[医药卫生—临床医学]