大鼠实验性脾虚证小肠粘膜内分泌细胞的免疫组织化学研究  

Immunohistochemical Study on the Enteroendocrine Cells of Small Intestine in Experimental Spleen Deficiency Syndrome in Rats

作　　者：李玲凤[1] 王秀琴[1] 杨进[1] 刘英琴[1] 韩秀兰[1] 刘绍杰[1] 

机构地区：[1]首都医学院组织胚胎教研室,北京100054

出　　处：《中国中西医结合杂志》1992年第8期483-484,共2页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：成年雄性大鼠50只,分为正常组、脾虚组、自然恢复组和治疗组。观察小肠粘膜一般组织结构及粘膜内胃泌素细胞和5-羟色胺细胞的变化。结果表明:粘膜未见明显异常改变。脾虚组胃泌素细胞和5-羟色胺细胞分泌功能活跃,这可能与脾虚证候发生有关。经四君子汤治疗后,治疗组+++的胃泌素细胞数比自然恢复组更接近正常,说明四君子汤对脾虚证有一定治疗作用。50 adult Wistar male rats were used and divided into 4 groups, i. e. normal control group,experimental Spleen Deficiency group induced by rhubarb, spontaneous recovery group and therapeutic group treated with Chinese recipe (Si Jun Zi decoction,四君子汤). All the animals of the 4 groups were killed simultaneously, and the jejunum and ileum were removed and processed for demonstration of gastrin cells and 5-HT cells according to immunohistochemical PAP technique. In adition, HE stained samples were prepared. The immunoreactivities of the two types of enteroendocrine cells were observed and semiquantitative estimation were performed under light microscopy.In addition, the immunoreactivities of 5-HT cells iri normal control and experimental Spleen Deficiency group were measured by microspectrocytophotometer (MPV 2, Leitz). All the data were treated statistically. This study revealed that there were no obvious histological changes in the mucosa among the 4 groups. In the jejunum, the percentage of gastrin cells (+) in experimental Spleen Deficiency group was more than that of the normal control group, while the percentage of gastrin cells (+ + +) was less than that of the the normal control group. As compared with spontaneous recovery group, it showed contrary to the above result in the therapeutic group. No gastrin cells were found in the ileum in all the 4 groups. the percentage of 5-HT cells did not show significant changes in the jejunum and ileum among the 4 groups. But immuno-reactivity in the 5-HT cell was less than that of the normal control group in the jejunum of the Spleen Deficiency group. The study suggested that the changes of percentage of gastrin cells and 5-HT cells in small intestine might be closely related to digestive disorders presented in Spleen Deficiency Syndrome and the Chinese recipe (Si Jun Zi decoction) currently used had significant therapeutic effect.

关 键 词：脾虚 免疫组织化学 

分 类 号：R229[医药卫生—中医基础理论]