包含YMDD基因序列的乙型肝炎病毒P区基因变异的研究  

A study on the mutation of P gene region including YMDD motif in hepatitis B virus

作　　者：封波[1] 魏来[2] 陈明[1] 李秀华[1] 

机构地区：[1]江苏徐州医学院附属医院传染科,徐州221002 [2]北京大学人民医院,北京大学肝病研究所

出　　处：《中华肝脏病杂志》2004年第1期29-31,共3页Chinese Journal of Hepatology

基　　金：江苏省教育厅自然科学研究基金(02KJD320035)

摘　　要：目的 研究包含YMDD基因序列的乙型肝炎病毒(HBV)P区基因的一级结构及其变异特点。 方法 从4例未经任何抗病毒治疗的HBV感染患者外周血血清中扩增HBV P基因区序列(长度为1057 bp),双酶切后克隆入pUC19载体中,每份标本随机取20个经限制性片段长度多态性分析(RFLP)法鉴定为阳性的克隆,采取错配聚合酶链反应-限制性片段长度多态性分析(PCR—RFLP)法检测YMDD基序变异,最后取其中两个标本共10个阳性克隆进行双向测序。 结果 标本1和2克隆间核苷酸变异率分别为0.3％～1.1％,0.4％～1.7％。80个阳性克隆中,有2个克隆YMDD基序变异为YMGD。 结论 包含YMDD基序的HBV P区存在准种分布特征,在未经抗病毒治疗的患者血清中,存在一种新的YMDD变异形式,即YMGD变异。Objective To investigate the primary structure and heterogenenity of P gene region including YMDD motif in hepatitis B virus. Methods From serum samples collected from 4 patients who had never been treated with anti-viral drugs, DNA fragments of 1057 bp long of P gene were amplified and cloned into pUC19. Twenty positive clones were chosen randomly from each sample. The YMDD motif mutation was detected by mismatched PCR-RFLP. Finally, last ten positive clones of two samples were sequenced. Results Nucleotide mutation rates among clones of Sample 1 and 2 were 0.3%-1.1%, 0.4%-1.7%, respectively. Among 80 clones, the variations from YMDD to YMGD were revealed in two clones. Conclusion There are HBV quasispecies in the P gene region including YMDD motif of hepatitis B virus and a novel mutation of YMDD motif in the sera of patients without being therapied by anti-viral drugs.

关 键 词：YMDD基因 基因序列 乙型肝炎病毒 HBV 基因变异 肝脏损伤 

分 类 号：R512.62[医药卫生—内科学]