阴道分泌物白细胞介素8与孕妇下生殖道感染关系的研究  

Relationship between concentration of IL-8 in vaginal discharge and adverse pregnancy outcomes in women with lower genital tract infection

作　　者：任平[1] 祝杏珍[2] 官洁[1] 林克萍[2] 陈天星[3] 董旭东[2] 陆坚贞[1] 贺静[1] 

机构地区：[1]云南省第一人民医院生殖遗传科,昆明650032 [2]云南省第一人民医院产科,昆明650032 [3]云南省第一人民医院病理科,昆明650032

出　　处：《中华围产医学杂志》2004年第2期79-82,共4页Chinese Journal of Perinatal Medicine

基　　金：云南省卫生厅 (2 0 0 0YWJM 10 )资助

摘　　要：目的　了解正常和下生殖道感染孕妇阴道分泌物白细胞介素 8(interleukin 8,IL 8)的水平变化特点与妊娠结局的关系。　方法　对下生殖道有霉菌、滴虫、沙眼衣原体、解脲支原体、人型支原体、细菌性阴道病感染或其中一种以上微生物感染的 2 5 5例观察组孕妇阴道分泌物进行IL 8定量检测及妊娠结局的随访观察 ,观察组中发生不良妊娠结局的孕妇取胚胎组织和胎盘组织进行病原体及病理检查 ,下生殖道无上述微生物感染的 12 0例正常孕妇作为对照组。　结果　 (1)观察A组在妊娠早、中、晚期IL 8浓度的平均值分别为 (2 2 2 .9± 193.6 )ng/L、(32 0 .0± 16 3.9)ng/L、(42 3.1± 115 .9)ng/L ,对照组IL 8浓度的平均值分别为 (12 5 .0± 4 5 .3)ng/L、(2 6 9.0± 5 9.5 )ng/L、(35 9.0± 10 7.1)ng/L ,两组比较差异有极显著性 (P <0 .0 1)。 (2 )IL 8>80 0ng/L的观察B组中用抗生素治疗的孕妇有 5 .2 6 %(2 / 38)发生异常妊娠结局 ,而未用抗生素治疗的孕妇有 78.13%(2 5 / 32 )发生异常妊娠结局 ,两组比较差异有极显著性 (P <0 .0 0 1)。 (3)妊娠结局异常孕妇的胚胎和胎盘组织中检出与宫颈分泌物相同的病原微生物 ,病理检测有绒毛膜炎性表现。　结论　ILObjective To investigate the relationship between concentrations of IL-8 in vaginal discharge and adverse pregnant outcome in women with lower genital tract infection. Methods Vaginal discharge was obtained from the observative group of 255 pregnant women with Fungal,Trichomonas vaginalis,Chlamydia trachomati (CT) ,Ureaplasma urealyticum (UU),Mycoplasma hominis(MH) or Bacteria vaginosis(BV). The concentrations of IL-8 were determined by enzyme-linked immunosorbent assay (ELISA). Pathological study and bacterial detection were performed in fetal and placental tissue in women with adverse pregnant outcomes . The control group included 120 pregnant women without lower genital tract infection. Results (1) The levels of IL-8 in observative group A in first,second and third trimester were (222.9±193.6)ng/L,(320.0±163.9) ng/L and (423.1±115.9)ng/L respectively. The levels of IL-8 in control group were (125.0±45.3) ng/L ,(269.0±59.5) ng/L and (359.0±107.1) ng/L respectively. There were significant differences between the two groups (P<0.01). (2) When the level of IL-8 increased over than 800 ng/L,78.13%(25/32) of the observation group B had adverse pregnant outcomes, while 5.26%(2/38) in the observation group B with antibiotic treatment. There were significant differences between these two groups (P<0.001). (3) The same microorganisms were detected in the tissues and the chorioamnionitis were proved by pathology. Conclusion The IL-8 concentrations of vaginal fluid has close relationship with lower genital tract infection and chorioamnionitis. The level of IL-8 in vaginal discharge can be used as a marker of subclinical intraamniotic infection of the pregnant women with lower genital tract infection.

关 键 词：阴道分泌物 白细胞介素8 孕妇 生殖道感染 

分 类 号：R714.25[医药卫生—妇产科学]