CD_(34)^+CD_(62L)^+细胞输入量对儿童急性白血病无关脐血移植的影响  

Effect of numbers of CD_(34)^+CD_(62L)^+cells infused on unrelated umbilical cord blood transplantation in children with acute leukemia

作　　者：吴韶清[1] 廖灿[1] 辜少玲[1] 陈劲松[1] 许遵鹏[1] 刘斌[1] 

机构地区：[1]广州市妇婴医院广州市脐血库,510180

出　　处：《中华儿科杂志》2004年第1期70-73,共4页Chinese Journal of Pediatrics

基　　金：广州市科技新星计划(1999 K 0 0 6 0 2 )

摘　　要：目的　研究CD+ 3 4 CD+ 62L细胞输入量在无关脐血移植治疗儿童急性白血病中对造血干细胞植入、中性粒细胞和血小板恢复时间的影响。方法　用流式细胞术分析复苏后的CD+ 3 4 CD+ 62L细胞数 ,并对 2 3例急性白血病儿童在无关脐血移植后的中性粒细胞和血小板恢复时间等临床资料进行测定。结果　 2 3例患儿中 ,2 1例被植入 ,其中性粒细胞 >5 0 0 / μl的时间为 11～ 2 3d(中位数为 17 5d) ,而在 18例患儿中 ,血小板 >2万 / μl的时间为 12～ 118d(中位数 4 1d)。当CD+ 3 4 CD+ 62L细胞输入量 >1 3× 10 5/kg时 ,有利于造血干细胞的植入 ,中性粒细胞的恢复时间与CD+ 3 4 CD+ 62L细胞输入量存在一定的相关性趋势 ,γ值为 - 0 32 4 ,0 0 5 <P <0 1,血小板恢复时间与CD+ 3 4 CD+ 62L细胞输入量有相关性 ,γ值为 - 0 4 70 ,P <0 0 5。结论　研究表明CD+ 3 4 CD+Objective Several studies have shown that L selectin on CD34 positive cells play a role in hematopoietic reconstitution after peripheral blood stem cell transplantation and allograft bone marrow transplantation This study sought to investigate whether the numbers of CD + 34 CD + 62L cells infused affect the engraftment of hematopoietic stem cells (HSC) and the time to neutrophil and platelet recovery after unrelated umbilical cord blood transplantation for the treatment of childhood acute leukemia Methods Twenty three children with acute leukemia who received unrelated umbilical cord blood transplantation of mostly mismatched HLA locus were included in this study Flow cytometry was used to count the numbers of CD + 34 CD + 62L cells after freezing thawing by labelling the cells with anti CD 34 and anti CD62L The patients′ clinical data including body weight, engraftment of the HSC, times to neutrophil and platelet recovery were evaluated Results Twenty one patients who received CD + 34 CD + 62L cell infusion at a number ranging from 1 37×10 5/kg to 2 68×10 6/kg (median, 3 567×10 5/kg) had successful engraftment of the unrelated umbilical HSC The numbers of CD + 34 CD + 62L cells infused were statistically different between patients who had successful engraftment of the umbilical HSC and those who had not ( P <0 05) The engraftment occurred more commonly in patients who received >1 3×10 5 CD + 34 CD + 62L cells/kg The time of neutrophil recovery (>500/μl) ranged from 11 days to 32 days (median, 17 5 days) The data of the time to platelet recovery (>2×10 5/μl) were obtained in 18 patients, and it ranged from 12 days to 118 days (median, 14 days) There seemed to be a tendency of correlation between the numbers of CD + 34 CD + 62L cells infused and time to platelet recovery (γ=-0 324,0 05< P <0 1), whereas the numbers of CD + 34 CD + 62L cells infused correlated with the time to platelet recover

关 键 词：细胞输入量 儿童 急性白血病 脐血移植 造血干细胞 L选择素 

分 类 号：R733.71[医药卫生—肿瘤]