同源盒基因Pax-6在鼠晶状体上皮细胞中的表达分析  

Expression of Pax-6 homeobox gene in lens epithelial cells in vitro

作　　者：刘奕志[1] 夏朝霞[1] 柳夏林[1] 黄强[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《中华眼科杂志》2003年第7期395-399,共5页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 (30 0 70 80 2 ) ;教育部高等医学院校博士学科点专项科研基金资助项目 (9949)

摘　　要：目的　观察体外培养的小鼠晶状体上皮细胞 (LEC)内同源盒基因Pax 6的表达 ,探讨维持晶状体上皮细胞特性的基因因素。方法　体外培养小鼠原代、传 1代、传 2代及传 3代LEC ,利用免疫组织化学法和免疫印迹法 ,检测细胞中Pax 6基因的蛋白表达情况 ,利用逆转录聚合酶链反应法检测细胞中Pax 6基因的mRNA表达情况。结果　在原代、传 1代及传 2代培养的LEC中均可检测到Pax 6基因蛋白和mRNA的阳性表达 ,传 3代培养的LEC中仅检测到Pax 6基因蛋白的微弱表达。阴性对照均未见Pax 6基因的表达。结论　体外培养的小鼠LEC中具有Pax 6基因 ,该基因的正常表达是维持LEC特性的必要条件。Objective To detect the gene expression of Pax-6 in cultured lens epithelial cells(LECs)of mouse in vitro in order to study the gene factorthat maintains the LECs characteristics. MethodsCultured the LECs (passage 1 to 3) were used in the study, the expression of Pax-6 gene in LECs was demonstrated by reverse transcriptase polymerase chain reaction (RT-PCR), immunohistochemical staining and Western blot. Results The mRNA of Pax-6 gene was positive in primary culture, the first and second passage of LECs using RT-PCR. The protein of Pax-6 gene was also positive in Western blot and immunohistochemical staining,but it was weak positive in the third passage of LECs and none in all passages of negative control groups. Conclusion The LECs are positive for the gene of Pax-6. The normal expression of Pax-6 gene is vital for maintaining the LECs characteristics.

关 键 词：同源盒基因 PAX-6 鼠 晶状体 上皮细胞 基因表达 LEC 免疫组织化学 

分 类 号：R770.2[医药卫生—眼科]