肝素缓释系统抑制后发性白内障的实验研究  

Experimental study on the prevention of after cataract by heparin drug delivery system

作　　者：孙洁[1] 谢立信[1] 姚瞻[1] 

机构地区：[1]山东省眼科研究所,青岛266071

出　　处：《中华眼科杂志》2003年第7期406-410,共5页Chinese Journal of Ophthalmology

基　　金：山东省科技厅基金资助项目 (0 12 130 12 1)

摘　　要：目的　探讨肝素缓释系统抑制后发性白内障发生的可行性和有效性。方法　将 30只(30只眼 )超声乳化透明晶状体吸除术后新西兰大白兔随机均分为A组 (术后生理盐水滴眼 )、B组(术中后房内植入空白缓释系统 )及C组 (术中后房内植入肝素缓释系统 )。术后 12周对 3个组兔眼进行裂隙灯显微镜、组织病理学及电镜检查 ,并检测血、房水肝素浓度和晶状体后囊膜的湿重。结果(1)术后 12周A、B及C组发生晶状体后囊膜混浊的眼数分别为 10、10及 4只眼 ,差异有非常显著意义(χ2 =14 0 9,P =0 0 0 1)。 (2 )术后 12周A、B及C组晶状体后囊膜的平均湿重分别为 (15 8± 5 )、(16 0± 9)及(2 0± 3)mg,差异有非常显著意义 (F =116 8 99,P =0 0 0 0 )。 (3)C组房水中的平均肝素浓度为 (2 6± 12 )mg/L。 (4)光镜和电镜下C组兔眼晶状体后囊膜细胞增殖不活跃 ,未发现眼内毒性反应。 (5 )术中和术后无眼内出血现象。结论　后房内植入肝素缓释系统 ,可明显提高并长期维持房水中的肝素浓度 ,有效抑制后发性白内障的发生 ;该方法毒副作用小 ,是一种安全、有效的给药方式。Objective Heparin drug delivery system (HEP DDS) was developed and applied to the experimental animals for the prevention of after cataract. Methods Thirty New Zealand rabbits (30 eyes) received phacoemulsification, and were randomly divided into 3 groups: normal saline eye dripping (Group A); posterior chamber implantation of carrier drug delivery system (Group B) or HEP DDS (Group C). All eyes were examined by slit-lamp microscopy, the blood and aqueous humor heparin concentrations were measured and the wet weight of posterior capsules was weighed. Results (1) In the 12th week after the operation, the number of eyes developed posterior capsule opacification in Groups A, B, and C was 10, 10 and 4, respectively; with statistically very significant difference (χ 2=14.09, P=0.001). (2) Twelve weeks after operation, the average wet weight of the posterior capsules in these 3 groups was (158±5) mg, (160±9) mg and (20±3) mg, respectively; with statistically very significant difference (F=1 168.99, P=0.000). (3) The average value of the aqueous humor heparin concentration in group C was (26±12) mg/L. (4) Light and electron microscopy did not revealed any active cell proliferation in the posterior capsules in Group C; the conjunctiva, cornea, iris, ciliary body and retina remained intact, without obviously toxic reaction. (5) No intraocular hemorrhage occurred during the follow-up period. Conclusion The implantation of HEP DDS into the posterior chamber can raise the aqueous humor heparin concentration to a high level and remained for a long period. This procedure can effectively inhibit the occurrence of after cataract, has few toxic and side effects, and is proved to be a safe and effective procedure.

关 键 词：肝素缓释系统 后发性白内障 药物释放系统 并发症 手术摘除 

分 类 号：R779.66[医药卫生—眼科]